Anti-RANKL Antibody (12A668) recommended for detection of RANKL of mouse, rat and human origin by WB, IF and IHC(P)

Anti-RANKL Antibody (12A668): sc-52950

Anti-RANKL Antibody (12A668) is rated 4.5 out of 5 by 4.
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Datasheets

    • RANKL Antibody (12A668) is a mouse monoclonal IgG1, cited in 12 publications, provided at 50 µg/0.5 ml
    • raised against amino acids 1-317 of RANKL of mouse origin
    • recommended for detection of RANKL of mouse, rat and human origin by WB, IF and IHC(P)
    • See RANKL (G-1): sc-377079 for RANKL antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of RANKL (12A668): sc-52950.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for RANKL Antibody (12A668) for WB and IHC(P) applications. These reagents are now offered in bundles with RANKL Antibody (12A668) (see ordering information below).
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Can sc-52950: RANKL (12A668) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Professor Griffin
Thank you for your question. Yes, sc-52950: RANKL (12A668) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from 条带不错 Have a good job,but the results are not coherent to my thoughts.
Date published: 2017-11-10
Rated 4 out of 5 by from the result is acceptable Have a good job, the price is also very kind. I will finish my next work with it.
Date published: 2017-11-07
Rated 5 out of 5 by from Versatile Antibody This antibody is well-cited for a variety of applications including WB, and IHC. Strong, clear signal with little to no background. Beautiful tissue staining. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-02-08
Rated 4 out of 5 by from Publishable WB using MDA Publishable WB using MDA-MB-231, MCF7 human breast carcinoma cell, 4T1 murine breast carcinoma cell, MCF10A human mammary epithelial cells, MC3T3-E1 mouse preosteoblastic cell, and mouse-derived bone marrow cells - SCBT Publication Review
Date published: 2014-03-30
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