Anti-RANK Antibody (H-7) recommended for detection of RANK of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-RANK Antibody (H-7): sc-374360

Anti-RANK Antibody (H-7) is rated 4.6 out of 5 by 9.
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Datasheets

    • Anti-RANK Antibody (H-7) is a mouse monoclonal IgG1 κ RANK antibody, cited in 17 publications, provided at 200 µg/ml
    • raised against amino acids 317-616 of RANK of human origin
    • Anti-RANK Antibody (H-7) is recommended for detection of RANK of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-RANK Antibody (H-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of RANK (H-7): sc-374360.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RANK Antibody (H-7) for WB applications. These reagents are now offered in bundles with RANK Antibody (H-7) (see ordering information below).
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Can this antibody be used in Western Blotting of rabbit bone tissue?Thank you!

Asked by: jack35
Thank you for your question. This antibody does not have data or any product citations in rabbit samples at this time. Please contact our Technical Service Department if you would like our assistance in checking the antibody's antigen sequence for similarity with the RANK protein in rabbits.
Answered by: Technical Support
Date published: 2020-04-20

I plan to do the immonstaining of Rank+ cells in the bone. Can I apply for a sample of this antibody to do the experiment? Thank you.

Asked by: Weiping Su
Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2019-01-14

RANK抗体(H-7): sc-374360 を購入したいと考えています。日本円でのお値段を教えてください。

Asked by: Kazuhiro
Thank you for your question. If you would like to check the price in Japanese Yen(JPY), please contact one of our local distributors in Japan. https://www.scbt.com/scbt/resources/distributors Please feel free to contact me if I may be of further assistance. お問い合わせを頂きまして、誠にありがとうございました。日本での購入は弊社の日本での代理店へお勧めします。納期と日本円での価格は代理店へご連絡ください。 代理店は下記とな ります。 https://www.scbt.com/scbt/resources/distributors その他の不明点等がございましたら、またご連絡ください。
Answered by: Technical Support 8
Date published: 2018-12-21

Hi, Can i get this free sample antibody of RANK (H-7, sc-374360). i would like to test this antibody for our experiment. let me know. Thank you.

Asked by: Sehwan
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

Can RANK (H-7): sc-374360 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, RANK (H-7): sc-374360 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Western blotting testing for Mouse macrophage cell I detected the expression of RANK of mouse macrophage cells, and compare B-8 and H-7. The H-7 is good for deteting mouse macrophage cells by western blotting
Date published: 2019-03-15
Rated 4 out of 5 by from It can be used I applied for the sample of this product, and I got bands which were a little bit shallow.
Date published: 2018-05-06
Rated 4 out of 5 by from 实验结果理想 一个月前在贵公司申请免费抗体小样,该抗体效果还可以,值得购买,稀释比例大概是1:250,推荐过夜孵育,曝光时间长一点
Date published: 2017-10-16
Rated 5 out of 5 by from 1:600 dilution RANK is a good marker in bone metabolism testing. We detected the expression of RANKL in osteoclasts cell by RANKL antibody,recommend 1:600 dilution, the secondary from CWBIO, 1:3000 dilution, the target protein expression is very high.
Date published: 2017-09-16
Rated 5 out of 5 by from Great work well I bought RANK (H-7) a few month ago and I could improve my western bot than other RANK antibody
Date published: 2017-06-28
Rated 4 out of 5 by from Clear bands We got RANK antibody 3 months ago . It was applied to WB , the bands were clear , but the background was a bit high .
Date published: 2017-06-13
Rated 5 out of 5 by from Great for RANK Used for western blotting with mouse RANK transfected, the antibody is good to WB at dilution 1:1000.
Date published: 2017-02-12
Rated 5 out of 5 by from positive western blot results We used this antibody in a western blot and produced positive band results at the correct molecular weight
Date published: 2017-01-25
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