Anti-RANK Antibody (B-8) recommended for detection of RANK of mouse and rat origin by WB, IP, IF and ELISA

Anti-RANK Antibody (B-8): sc-390655

Anti-RANK Antibody (B-8) is rated 5.0 out of 5 by 4.
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Datasheets

    • RANK Antibody (B-8) is a mouse monoclonal IgG2a κ, cited in 4 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 587-612 near the C-terminus of RANK of mouse origin
    • Anti-RANK Antibody (B-8) is recommended for detection of RANK of mouse and rat origin by WB, IP, IF and ELISA
    • Anti-RANK Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for RANK Antibody (B-8) for WB applications. These reagents are now offered in bundles with RANK Antibody (B-8) (see ordering information below).
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What application is the blocking peptide sc-390655 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Works great! This antibody works very well on mouse calvariae at a 1:500 dilution for western blot.
Date published: 2018-03-16
Rated 5 out of 5 by from good chocie for mouse and rat RANK This monoclonal RANK Antibody (B-8) is a good choice for detection of mouse and rat RANK protein by IF or Western blotting.
Date published: 2017-02-03
Rated 5 out of 5 by from Produced beautiful immunofluorescence cytoplasmic Produced beautiful immunofluorescence cytoplasmic staining in methanol-fixed NIH/3T3 cells. -SCBT QC
Date published: 2015-04-16
Rated 5 out of 5 by from Produced positive Western Blot data of RANK Produced positive Western Blot data of RANK expression in non-transfected and mouse RANK transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-02-05
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