Anti-RAGE Antibody (A11) recommended for detection of natural and recombinant RAGE of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-RAGE Antibody (A11): sc-80652

Anti-RAGE Antibody (A11) is rated 4.6 out of 5 by 8.
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Datasheets

    • RAGE Antibody (A11) is a mouse monoclonal IgG2a κ, cited in 23 publications, provided at 200 µg/ml
    • raised against a truncated extracellular domain of RAGE of human origin
    • Anti-RAGE Antibody (A11) is recommended for detection of natural and recombinant RAGE of mouse, rat and human origin by WB, IP, IF and IHC(P); also reactive with additional species, including bovine
    • Anti-RAGE Antibody (A11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of RAGE (A11): sc-80652.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for RAGE Antibody (A11) for WB and IHC(P) applications. These reagents are now offered in bundles with RAGE Antibody (A11) (see ordering information below).
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Dear Santa Cruz, Are you able to tell me the amino acid sequence contained within the 'truncated extracellular domain' that was used as the immunogen for this antibody?

Asked by: THeP
Thank you for your question. RAGE Antibody (A11) is a mouse monoclonal IgG2a raised against a truncated extracellular domain of RAGE of human origin. According to Uniprot accession # Q15109, the extracellular domain corresponds with amino acids 23-342. The exact epitope recognized by this clone has not been determined.
Answered by: Technical Support
Date published: 2017-01-31

I would like to use RAGE Antibody (A11): sc-80652 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-80652 is not available directly conjugated, but we have an alternative RAGE antibody (sc-365154) that is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2017-01-30
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Rated 5 out of 5 by from specific band in WB It's hard to find a good quality antibody, this antibody detects RAGE protein at 1:800 dilution in WB assays . Works well in mouse kidney tissue , always works in every condition. Easy to use.
Date published: 2017-07-29
Rated 5 out of 5 by from excellent antibody Used with very good results in human colon tissue with IHC and cells
Date published: 2017-05-16
Rated 5 out of 5 by from strong antibody Brought up clear bands at the predicted molecular weight on western blots
Date published: 2017-04-17
Rated 5 out of 5 by from Well characterized RAGE ab for WB, FCM & IHC This antibody is well published in a variety of human tissue samples. Strong WB banding seen in OVCAR-3, HUV-EC-C, A549 and Jurkat whole cell lysates. and beautiful Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue.
Date published: 2017-01-06
Rated 4 out of 5 by from Various publishable data in WB using human Various publishable data in WB using human osteosarcoma cell lines KPDX, lung tissue and HUVECs. -SCBT Publication Review
Date published: 2015-05-18
Rated 5 out of 5 by from Great band seen in WB using IMR Great band seen in WB using IMR-32 whole cell lysate. -SCBT QC
Date published: 2015-05-09
Rated 4 out of 5 by from Worked with transfected lysate by WB Worked with transfected lysate by WB -SCBT QC
Date published: 2015-02-20
Rated 4 out of 5 by from Publishable data in FACS using human HUVECs Publishable data in FACS using human HUVECs. -SCBT Publication Review
Date published: 2014-07-09
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