Anti-Rad54B Antibody (19-K2) recommended for detection of Rad54B of human origin by WB, IP, IF and ELISA

Anti-Rad54B Antibody (19-K2): sc-101234

Anti-Rad54B Antibody (19-K2) is rated 4.0 out of 5 by 2.
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Datasheets

    • Anti-Rad54B Antibody (19-K2) is a mouse monoclonal IgG1 κ Rad54B antibody, cited in 3 publications, provided at 100 µg/ml
    • raised against recombinant Rad54B of human origin
    • recommended for detection of Rad54B of human origin by WB, IP, IF and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Rad54B (19-K2): sc-101234.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Rad54B Antibody (19-K2) for WB applications. These reagents are now offered in bundles with Rad54B Antibody (19-K2) (see ordering information below).
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I am using Rad54B (19-K2): sc-101234 for immunofluorescence, what dilution of this antibody should I use?

Asked by: AbPolly
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Band observed at correct molecular weight Band observed at correct molecular weight in Western Blot analysis with HeLa nuclear extract. -SCBT QC
Date published: 2015-03-20
Rated 4 out of 5 by from Produced wonderful immunofluorescence nuclear Produced wonderful immunofluorescence nuclear staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2014-06-18
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