Anti-Rad51 Antibody (G-5) recommended for detection of Rad51 of y and human origin by WB, IP, IF and ELISA

Anti-Rad51 Antibody (G-5): sc-133089

Anti-Rad51 Antibody (G-5) is rated 4.0 out of 5 by 3.
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Datasheets

    • Rad51 Antibody (G-5) is a mouse monoclonal IgG2a κ, cited in 5 publications, provided at 200 µg/ml
    • raised against amino acids 1-180 mapping at the N-terminus of Rad51 of Saccharomyces cerevisiae origin
    • Anti-Rad51 Antibody (G-5) is recommended for detection of Rad51 of y and human origin by WB, IP, IF and ELISA
    • Anti-Rad51 Antibody (G-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Rad51 (G-5): sc-133089.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Rad51 Antibody (G-5) for WB applications. These reagents are now offered in bundles with Rad51 Antibody (G-5) (see ordering information below).
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What is the recommended starting dilution for Western blot for sc-133089: Rad51 (G-5) monoclonal antibody?

Asked by: Cweed
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 3 out of 5 by from Works ok! We used this antibody for detecting Rad51 foci in response to DNA damagew by IF assay. We could observe the signal but no obvious foci.
Date published: 2018-03-27
Rated 4 out of 5 by from works for IF It can works in IF staining but the signal is not very strong, we use it in IF at 1:200.
Date published: 2017-09-13
Rated 5 out of 5 by from Strong Strong, clear band in WB in A-431 nuclear extract. -SCBT QC
Date published: 2013-01-18
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