Anti-Rad51 Antibody (F-11) recommended for detection of Rad51 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Rad51 Antibody (F-11): sc-398587

Anti-Rad51 Antibody (F-11) is rated 3.2 out of 5 by 19.
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Datasheets

    • Anti-Rad51 Antibody (F-11) is a mouse monoclonal IgG1 κ Rad51 antibody, cited in 23 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 311-339 at the C-terminus of Rad51 of human origin
    • Anti-Rad51 Antibody (F-11) is recommended for detection of Rad51 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Rad51 Antibody (F-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Rad51 (F-11): sc-398587.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Rad51 Antibody (F-11) for WB and IHC(P) applications. These reagents are now offered in bundles with Rad51 Antibody (F-11) (see ordering information below).
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Hello, I am thinking of trying sc-398587 Rad51 F-11 for IF in human cancer cells but I see some bad reviews for IF (and WB). Do you have protocol recommendations (as far as pre-extracting or not etc)? Thank you.

Asked by: adkjfd
Thank you for your question. sc-398587 is a great antibody for Rad51. It is recommended and warrantied for IF on human, mouse, and rat samples. Here is a recent publication using it for IF on human samples (PMID: 29694959). Here is a link to the recommended protocol. We recommend a starting dilution of 1:50. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Tech Service
Date published: 2019-08-30

If I want to test the specificity of RAD51, can I also have a sample of the blocking peptide sc-398587 P? Please let me know. Thanks.

Asked by: harias
Thank you for your question. We do not provide sample sizes of the blocking peptides.
Answered by: Technical Support
Date published: 2019-05-25

Do you have the one which can be used for the flow cytometry?

Asked by: ning25348
Thank you for your question. Unfortunately we do not offer an antibody that has been tested for Flow cytometry against Rad51, so they can not be recommended or warrantied for that use. Please contact Technical Service if you have any further questions.
Answered by: Tech Service
Date published: 2019-01-14

How can I get a trial sample for the monoclonal Ab Rad51 (F-11): sc-398587? Please let me know.

Asked by: harias
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

What is the recommended antigen retrieval method when doing IHC(P) with this antibody?

Asked by: AbPolly
Thank you for your question. We recommend using sodium citrate buffer, pH 6.0, and heat for antigen retrieval. The full recommended protocol can be found on our website here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-01-30
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Rated 1 out of 5 by from very very bad no band (different size band coming) and no IF staining. very bad
Date published: 2018-09-05
Rated 1 out of 5 by from Didn't work in my hands. Tried for western at 1:500 dilution and didn't detect any band in human neuroblastoma cancer cells. The discontinued works perfectly fine in the same lysates.
Date published: 2018-06-26
Rated 1 out of 5 by from Tried twice for WB, just got a smeary mess. Tried this as a sample, very disappointed. I firstly tried 1:100 5% milk in TBST, incubated for 1.5 hours at room temperature. I got nothing but smears on the blot. I then tried 1:500 incubation for 1.5 hours at room temperature followed by overnight incubation at 4 oC. This is the blot shown in he picture. Nothing at 37kDa and lots of messy smears, does not work at all. Loaded 30ug protein per well with llamaeli buffer. Normally works very well. GAPDH control (not shown) also worked well.
Date published: 2018-01-25
Rated 5 out of 5 by from 这个抗体和之前一样好用! 多年前买过santa cruz抗体,质量很好,物美价廉。这次试用的抗体一如既往的好用,以后会继续关注santa cruz。
Date published: 2017-12-19
Rated 5 out of 5 by from Good antibody I tried this antibody for Rad51. It is very good! The blot is very clean and clear. The cell line is U2OS. The middle lane is from cells infected by Rad51 shRNA.
Date published: 2017-11-04
Rated 3 out of 5 by from can't recognize any band in NPC cell lysates can't recognize any band in NPC cell lysates,1:250 in 5%TBST,4℃ overnight
Date published: 2017-09-22
Rated 5 out of 5 by from Great for WB Works great for WB at 1:1000 dilution in HeLa and 293T cells.
Date published: 2017-09-06
Rated 1 out of 5 by from It is not good for IF. Tried this antibody, but there was no signal at 1:50 diluted.
Date published: 2017-08-02
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