Anti-Rad51 Antibody (3C10) recommended for detection of Rad51 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Rad51 Antibody (3C10): sc-53428

Anti-Rad51 Antibody (3C10) is rated 3.8 out of 5 by 14.
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Datasheets

    • Rad51 Antibody (3C10) is a mouse monoclonal IgG1 λ, cited in 17 publications, provided at 200 µg/ml
    • raised against full length recombinant human RAD51 protein
    • Anti-Rad51 Antibody (3C10) is recommended for detection of Rad51 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Rad51 Antibody (3C10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Rad51 (3C10): sc-53428.
    • m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for Rad51 Antibody (3C10) for WB and IHC(P) applications. This reagent is now offered in a bundle with Rad51 Antibody (3C10) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Why are two bands obtained in the western blot? is it nonspecific?

Asked by: ISMS
Thank you for your question. These bands are not unspcific signals. Rad51 has four described isoforms of different molecular weights (37 kDa, 26 kDa, 31 kDa, and 36 kDa, respectively). When different isoforms are present in a given sample, the antibody will detect multiple specific bands.
Answered by: Technical Support Europe
Date published: 2020-10-08

Which is the epitope recognized by this antibody?

Asked by: UOGL
Thank you for your question. Rad51 Antibody (3C10): sc-53428 was raised against full length recombinant human RAD51 protein. So far, no epitope mapping has been performed for this antibody, so its exact epitope is not yet known.
Answered by: Tech Support Europe
Date published: 2019-05-09

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Rad51 Antibody (3C10): sc-53428?

Asked by: Trav11
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 15
Date published: 2017-01-24
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Rated 5 out of 5 by from Great for WB in the mouse samples I got the free sample and tested in WB using lysates from mouse brains and mouse cell line N2A. The antibody gives strong signals with generally clean background. and I have ordered this antibody for the future use. Generally, I loaded 15 μg protein for each sample and used 1:1000 antibody dilution in 5% BSA/TBST for the membrane incubation. For the signal detection, I used pico PLUS ECL from Thermo fisher to develop the signals and detected using X-ray films.
Date published: 2021-06-13
Rated 5 out of 5 by from Works for western blot Used for western blot on ovcar3 cells. Able to get detection at 1:500 in BSA
Date published: 2021-03-26
Rated 3 out of 5 by from Segnale discreto Ho provato questo anticorpo su vescicole extracellulari e loro cellula sorgente. Ho rilevato il segnale nelle cellule, ma solo con ECL ultra.
Date published: 2021-01-25
Rated 1 out of 5 by from not suitable for IF and WB we used this antibody in different concentrations for IF (dilution 1:50 - 1:500, cells were fixed with either formaldehyde or methanol) and WB (dilution range 1:50 - 1:1000 in BSA/TBS-T or MMP/TBS-T) but recieved no usable signals
Date published: 2020-01-09
Rated 1 out of 5 by from Not good for IF in my hands The antibody was used for immunofluorescence analysis on murine paraformaldehyde-fixed ovarian cells, but there was no signal at 1:100 and 1:50 dilution.
Date published: 2019-10-20
Rated 3 out of 5 by from General product When western blot is using this antibody, the antibody has a bad specificity and a mixed band
Date published: 2017-12-18
Rated 3 out of 5 by from WB in T98G cells It is ok this antibody. It shows non specific bands but still you can guess which band is the one you are looking for just looking at the molecular weight. I used this antibody in T98G cells lysis, dilution of the antibody 1:200 in 5% milk-PBSTween incubating over night
Date published: 2017-12-11
Rated 5 out of 5 by from Good western blot results in human Good western blot results in human.
Date published: 2015-03-02
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