Rad50 Antibody (G-2)recommended for detection of Rad50 of mouse, rat and human origin by WB, IP, IF and ELISA

Rad50 Antibody (G-2): sc-74460

Rad50 Antibody (G-2) is rated 4.7 out of 5 by 15.
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Datasheets

    • Rad50 Antibody (G-2) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1013-1312 mapping at the C-terminus of Rad50 of human origin
    • Rad50 Antibody (G-2) is recommended for detection of Rad50 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Rad50 Antibody (G-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Rad50 (G-2): sc-74460.
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What is the recommended fixation method for immunofluorescence staining with Rad50 (G-2): sc-74460 monoclonal antibody?

Asked by: Trav11
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from OK for western blot OK for western blot (1:1000 dilution) in lung cancer cell lines
Date published: 2018-07-19
Rated 5 out of 5 by from Good for WB I used this antibody for my WB on MDA-MB231 cells. It gives me clean bands with a non specific bands below but no background.
Date published: 2018-02-01
Rated 5 out of 5 by from Great antibody Nice and strong by wb detection in MCF7 cell lines
Date published: 2017-11-29
Rated 5 out of 5 by from Good Worked for our rat cell line. Used at 1:1000 for WB. Good signal.
Date published: 2017-11-15
Rated 5 out of 5 by from Nice results by WB on mouse colon extracts We were able to detect the band at the proper MW on mouse colon extracts challenged by DNA damage. We used regular ECL and a 1/500 dilution for the ab. Saturation of the membrane and incubation with the ab was performed in TBS-Tween-5% non fat milk. The signal showed up after around 10 min of revelation
Date published: 2017-10-27
Rated 5 out of 5 by from Good one The sample antibody worked well for WB in HEK-293T cells at 1:500 dilution.
Date published: 2017-08-16
Rated 1 out of 5 by from This antiody did not work at 1:1000 in WB of U2OS This antibody did not work at 1:1000 in WB of U2OS cells.
Date published: 2017-07-05
Rated 5 out of 5 by from High Quality Product The Rad50 Antibody (G-2): sc-74460 provides excellent data for immunofluorescence applications showing staining pattern with nuclear localization. This antibody has also been cited multiple times for further verification.
Date published: 2017-02-03
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