Anti-Rad17 Antibody (H-3) recommended for detection of Rad17 of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Rad17 Antibody (H-3): sc-17761

Anti-Rad17 Antibody (H-3) is rated 5.0 out of 5 by 7.
  • y_2022, m_8, d_17, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.32
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_en_US, sid_17761, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 114ms
  • REVIEWS, PRODUCT
loading
5
7
4
0
3
0
2
0
1
0

Datasheets

    • Anti-Rad17 Antibody (H-3) is a mouse monoclonal IgG1 κ Rad17 antibody, cited in 13 publications, provided at 200 µg/ml
    • raised against amino acids 371-670 of Rad17 of human origin
    • Rad17 Antibody (H-3) is recommended for detection of Rad17 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Rad17 Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Rad17 (H-3): sc-17761.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Rad17 Antibody (H-3) for WB and IHC(P) applications. These reagents are now offered in bundles with Rad17 Antibody (H-3) (see ordering information below).
loading

I am using Rad17 (H-3): sc-17761 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
  • y_2022, m_8, d_18, h_20CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.32
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_17761, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 117ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Great antibody for IF! I bought this antibody a few days ago and It works really well in IF in PFA fixed cells. Dilution 1:100
Date published: 2022-03-31
Rated 5 out of 5 by from good Worked very well for WB of primary human keratinocyte.
Date published: 2017-02-09
Rated 5 out of 5 by from Good WB publishable data gathered with human Good WB publishable data gathered with human 293A cell. -SCBT Publication Review
Date published: 2015-04-03
Rated 5 out of 5 by from Good specificity and signal strength with Good specificity and signal strength with some background bands in HeLa and Jurkat nuclear extracts.-SCBT QC
Date published: 2015-03-27
Rated 5 out of 5 by from Good specificity and signal strength in non Good specificity and signal strength in non-transfected HeLa cells. Actin (I-19) used as specificity and loading control. -SCBT
Date published: 2015-03-01
Rated 5 out of 5 by from Works well for WB with non Works well for WB with non-transfected 293T, human Rad17 transfected 293T and HeLa whole cell lysates. -SCBT QC
Date published: 2015-01-29
Rated 5 out of 5 by from Superb immunoperoxidase nuclear staining Superb immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human testis cancer. -SCBT QC
Date published: 2013-01-02
  • y_2022, m_8, d_17, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.32
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_en_US, sid_17761, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 27ms
  • REVIEWS, PRODUCT