Rad17 Antibody (1C6/2)recommended for detection of Rad17 of yeast origin by WB

Rad17 Antibody (1C6/2): sc-53427

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Datasheets

    • Rad17 Antibody (1C6/2) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against a Rad17 fusion protein of S. pombe origin
    • recommended for detection of Rad17 of yeast origin by WB
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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How much sample do you recommend loading on the gel for Western blot detection using Rad17 (1C6/2): sc-53427?

Asked by: Germaine
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-27
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