Anti-Rab 7b Antibody (JK-17) recommended for detection of Rab 7b of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Rab 7b Antibody (JK-17): sc-81922

Anti-Rab 7b Antibody (JK-17) is rated 5.0 out of 5 by 4.
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Datasheets

    • Anti-Rab 7b Antibody (JK-17) is a mouse monoclonal IgG2a κ Rab 7b antibody, cited in 4 publications, provided at 100 µg/ml
    • raised against recombinant Rab 7b of human origin
    • recommended for detection of Rab 7b of human origin by WB, IP, IF, IHC(P) and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Rab 7b (JK-17): sc-81922.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Rab 7b Antibody (JK-17). This work is in progress.
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Can sc-81922: Rab 7b (JK-17) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenniferc
Thank you for your question. Yes, sc-81922: Rab 7b (JK-17) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from western blot HEK細胞を用いて通常のウエスタンブロットを行い、予想される高さにクリアなシングルバンドを認めました。
Date published: 2018-10-24
Rated 5 out of 5 by from Worked for IF with paraformaldehyde Worked for IF with paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-04-04
Rated 5 out of 5 by from Robust staining in paraffin Robust staining in paraffin-embedded human small Intestine tissue. -SCBT QC
Date published: 2015-02-02
Rated 5 out of 5 by from Very nice looking Western using HeLa whole Very nice looking Western using HeLa whole cell lysate. -SCBT QC
Date published: 2014-09-20
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