Anti-Rab 7 Antibody (B-3) recommended for detection of Rab 7 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-Rab 7 Antibody (B-3): sc-376362

Anti-Rab 7 Antibody (B-3) is rated 4.3 out of 5 by 27.
  • y_2021, m_10, d_27, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_26
  • loc_en_US, sid_376362, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 82ms
  • REVIEWS, PRODUCT
loading
5
13
4
10
3
3
2
1
1
0

Datasheets

    • Anti-Rab 7 Antibody (B-3) is a mouse monoclonal IgG1 κ Rab 7 antibody, cited in 50 publications, provided at 200 µg/ml
    • raised against amino acids 158-207 mapping at the C-terminus of Rab 7 of human origin
    • Anti-Rab 7 Antibody (B-3) is recommended for detection of Rab 7 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-Rab 7 Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Rab 7 (B-3): sc-376362.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Rab 7 Antibody (B-3) for WB applications. These reagents are now offered in bundles with Rab 7 Antibody (B-3) (see ordering information below).
loading

I can not detect Rab7 in mouse motor cortex with this antibody. Have any of you tested in neuron primary culture?

Asked by: JáinaR
Thank you for your question. We have not tested this antibody in neuron primary culture.
Answered by: Technical Support
Date published: 2019-07-14

Can you recommend a positive control to use with Rab 7 Antibody (B-3): sc-376362 for Western blot?

Asked by: cjMara
We recommend C2C12 (sc-364188), 3T3-L1 (sc-2243), RAW 264.7 (sc-2211) or A549 (sc-2413) whole cell lysates to use as positive controls as we have Western blot data showing specific detection of Rab 7 in these cell lines.
Answered by: Technical Support 9
Date published: 2017-01-09
  • y_2021, m_10, d_27, h_19CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_376362, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 85ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Good for IF マウスの培養細胞を用いてPFA固定後に免疫蛍光染色を行いました。エンドゾーム様の染色像を確認することができました。
Date published: 2020-08-24
Rated 5 out of 5 by from great Clear crisp bands, only one band, at predicted molecular weights
Date published: 2020-02-06
Rated 4 out of 5 by from worked great for IF for murine dendritic cells worked great for IF for murine dendritic cells thank you
Date published: 2019-12-04
Rated 4 out of 5 by from Good antibody This antibody works well for WB in HeLa and COS7 cells (1:500)
Date published: 2019-06-19
Rated 4 out of 5 by from GOOD It worked well in 1:3000 in our cell lines, and I had pretty good signals
Date published: 2018-04-03
Rated 5 out of 5 by from Rab7 (B3) works very well Antibody works very well for HeLa WB (1/500 dil 4°C O/N) and IF (1/50).
Date published: 2018-02-25
Rated 4 out of 5 by from For IF with Rab 7 In HeLa cell lines worked fine with antibody dilution 1:50
Date published: 2018-02-13
Rated 5 out of 5 by from Works on blots from rat neurons I received a sample of this antibody and tried it on an immunoblot of rat primary neurons. We ran 20 ug of lysate and used the antibody at 1:200 (stock concentration as supplied =0.2 ug/ul). The blots were very clean and Rab7 stained extremely well.
Date published: 2018-01-17
  • y_2021, m_10, d_27, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_26
  • loc_en_US, sid_376362, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT