Anti-Rab 5B Antibody (F-9) recommended for detection of Rab 5B of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-Rab 5B Antibody (F-9): sc-373725

Anti-Rab 5B Antibody (F-9) is rated 5.0 out of 5 by 5.
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Datasheets

    • Anti-Rab 5B Antibody (F-9) is a mouse monoclonal IgG1 κ Rab 5B antibody, cited in 4 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 186-219 within the C-terminus of Rab 5B of human origin
    • Anti-Rab 5B Antibody (F-9) is recommended for detection of Rab 5B of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine and porcine
    • Anti-Rab 5B Antibody (F-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Rab 5B (F-9): sc-373725.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Rab 5B Antibody (F-9) for WB applications. These reagents are now offered in bundles with Rab 5B Antibody (F-9) (see ordering information below).
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What application is the blocking peptide sc-373725 P appropriate for?

Asked by: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent! This antibody is great in western blot analysis of Rab 5B expression in NIH/3T3 whole cell lysate.
Date published: 2017-02-10
Rated 5 out of 5 by from good. Western blot, 1:1000, loading 20ug, in AsPC-1 & MIA PaCa-2 Cell lines. Got good band.
Date published: 2017-02-06
Rated 5 out of 5 by from very good. We asked free test sample from Santa and got good band for western blot. Then we buy a new one. Good antibody. Loading 10-20ug, 1:1000 dilution, easy to get strong band in HEK293T, AsPC-1, MIA PaCa-2 cell lines.
Date published: 2016-12-10
Rated 5 out of 5 by from Very clear and single band in NIH/3T3 whole Very clear and single band in NIH/3T3 whole cell lysate by WB. -SCBT QC
Date published: 2014-02-21
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