Anti-Rab 5 Antibody (D-11) recommended for detection of Rab 5A, 5B and 5C; partially cross reactive with other Rab family members of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Rab 5 Antibody (D-11): sc-46692

Anti-Rab 5 Antibody (D-11) is rated 4.4 out of 5 by 34.
  • y_2021, m_9, d_16, h_2
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_34
  • loc_en_US, sid_46692, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 8ms
  • REVIEWS, PRODUCT
loading
5
23
4
7
3
1
2
0
1
3

Datasheets

    • Anti-Rab 5 Antibody (D-11) is a mouse monoclonal IgG2b κ Rab 5 antibody, cited in 89 publications, provided at 200 µg/ml
    • raised against amino acids 1-215 representing full length Rab 5AA of human origin
    • Anti-Rab 5 Antibody (D-11) is recommended for detection of Rab 5A, 5B and 5C; partially cross reactive with other Rab family members of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine
    • Anti-Rab 5 Antibody (D-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Rab 5 (D-11): sc-46692.
    • m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Rab 5 Antibody (D-11) for WB and IHC(P) applications. These reagents are now offered in bundles with Rab 5 Antibody (D-11) (see ordering information below).
loading

I see that the Rab 5 Antibody (D-11) is reactive with multiple species. Will it work with bovine samples?

Asked by: Trav11
To check additional species reactivity not listed on our website, please contact our Technical Services department at 800-457-3801 and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Tech Service 8
Date published: 2016-12-20

Is it possible to use the Rab 5 Antibody (D-11) for double staining if my other primary antibody was also raised in mice?

Asked by: Trav11
Yes, it is possible. For cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, the Rab 5 Antibody (D-11): sc-46692 is available conjugated to HRP (sc-46692 HRP), phycoerythrin (sc-46692 PE), fluoroscein (sc-46692 FITC), Alexa Fluor® 488 (sc-46692 AF488) or Alexa Fluor® 647 (sc-46692 AF647).
Answered by: Tech Service 8
Date published: 2016-12-20
  • y_2021, m_9, d_16, h_2CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_46692, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 9ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Works very well in Western and IF Works well in Western blot and immunofluorescence.
Date published: 2021-01-13
Rated 5 out of 5 by from great for wb I got the sample size and it worked great in the WB.
Date published: 2020-07-08
Rated 5 out of 5 by from excellent One clear crisp band, predicted molecular weights using concentration of 1:1000
Date published: 2020-02-06
Rated 5 out of 5 by from Nice staining in IF for early endosome we tested Rab 5 with IF in the cell lines and worked quite well at 1:500 4 C overnight and second AB RT 1h.
Date published: 2018-05-17
Rated 3 out of 5 by from WB Rab 5 (D-11) did not worked in the WB of mouse lung primary fibroblast. We used anti-mouse IgG as the secondary antibody. Maybe the problem was the secondary antibody.
Date published: 2018-03-28
Rated 1 out of 5 by from 免疫染色 固定した繊維芽細胞の免疫染色に使用しました。通常通り1:400に希釈して使用しましたが良い染色性は得られま戦でした。
Date published: 2018-03-22
Rated 5 out of 5 by from Good antibody for WB One band detected at right size. Dilution used: 1/1000. Block with 2% BSA
Date published: 2018-03-07
Rated 5 out of 5 by from Excelent Antibody! Excellent antibody for WB. Dilution 1/500. Incubation overnight at 4C in blocking solution (3%BSA+2%milk). Hela229 cells.
Date published: 2018-01-12
  • y_2021, m_9, d_16, h_2
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_34
  • loc_en_US, sid_46692, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT