Anti-Rab 4/14 Antibody (F-10) recommended for detection of Rab 4A, Rab 4B and Rab 14 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Rab 4/14 Antibody (F-10): sc-376243

Anti-Rab 4/14 Antibody (F-10) is rated 4.3 out of 5 by 6.
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Datasheets

    • Anti-Rab 4/14 Antibody (F-10) is a mouse monoclonal IgG2a κ Rab 4/14 antibody, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 1-213 representing full length Rab 4A of human origin
    • Anti-Rab 4/14 Antibody (F-10) is recommended for detection of Rab 4A, Rab 4B and Rab 14 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine; may cross-react with other Rab family members
    • Anti-Rab 4/14 Antibody (F-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Rab 4/14 (F-10): sc-376243.
    • m-IgG2a BP-HRP is the preferred secondary detection reagent for Rab 4/14 Antibody (F-10) for WB and IHC(P) applications. This reagent is now offered in a bundle with Rab 4/14 Antibody (F-10) (see ordering information below).
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I am using Rab 4/14 (F-10): sc-376243 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Trav11
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Did not work in human skeletal muscle Received a sample of Rab4/14 and used in human skeletal muscle tissue, whole cell lysate. This antibody did not perform well in this source. There was some very weak non-specific banding much higher than the indicated target size of 24kDa, but no other signal appeared even close to target. Primary was used at 1:100 overnight and secondary was Santa Cruz anti-mouse, sc-516102 at 1:2000.
Date published: 2018-05-17
Rated 5 out of 5 by from Excellent! This antibody works great in western blot analysis of Rab 4/14 expression in HeLa and Hep G2 whole cell lysates.
Date published: 2017-02-07
Rated 5 out of 5 by from Well enough cytoplasmic staining in paraffin Well enough cytoplasmic staining in paraffin-embedded human bone marrow tissue . -SCBT QC
Date published: 2015-05-03
Rated 5 out of 5 by from Good specificity and signal strength for Good specificity and signal strength for WB in HeLa (A) and Hep G2 (B) whole cell lysates. -SCBT QC
Date published: 2015-01-14
Rated 5 out of 5 by from Great membrane and cytoplasmic staining with Great membrane and cytoplasmic staining with methanol-fixed HeLa cells by IF. -SCBT QC
Date published: 2014-08-02
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