Anti-Rab 33A/B Antibody (D-12) recommended for detection of Rab 33A and Rab 33B of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-Rab 33A/B Antibody (D-12): sc-271199

Anti-Rab 33A/B Antibody (D-12) is rated 4.5 out of 5 by 2.
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Datasheets

    • Anti-Rab 33A/B Antibody (D-12) is a mouse monoclonal IgG2b κ Rab 33A/B antibody, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 35-75 mapping near the N-terminus of Rab 33A/B of human origin
    • Anti-Rab 33A/B Antibody (D-12) is recommended for detection of Rab 33A and Rab 33B of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-Rab 33A/B Antibody (D-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Rab 33A/B (D-12): sc-271199.
    • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Rab 33A/B Antibody (D-12) for WB applications. These reagents are now offered in bundles with Rab 33A/B Antibody (D-12) (see ordering information below).
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What is the recommended starting dilution for Western blot for sc-271199: Rab 33A/B (D-12) monoclonal antibody?

Asked by: Germaine
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 4 out of 5 by from anti-Rab33A/B (D12) This antibody works perfectly in Western blotting analysis at a 1:500 dilution.
Date published: 2018-05-03
Rated 5 out of 5 by from Great bands seen in Western Blot using U Great bands seen in Western Blot using U-937, Jurkat, HuT 78 and Raji whole cell lysates -SCBT QC
Date published: 2014-03-11
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