Anti-Rab 27a Antibody (E-8) recommended for detection of Rab 27a of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Rab 27a Antibody (E-8): sc-74586

Anti-Rab 27a Antibody (E-8) is rated 4.8 out of 5 by 6.
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Datasheets

    • Anti-Rab 27a Antibody (E-8) is a mouse monoclonal IgG2b κ Rab 27a antibody, cited in 10 publications, provided at 200 µg/ml
    • raised against amino acids 162-221 mapping at the C-terminus of Rab 27a of human origin
    • Anti-Rab 27a Antibody (E-8) is recommended for detection of Rab 27a of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Rab 27a Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Rab 27a (E-8): sc-74586.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for Rab 27a Antibody (E-8) for WB and IHC(P) applications. This reagent is now offered in a bundle with Rab 27a Antibody (E-8) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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What is the recommended concentration of Rab27a for IHC(FFPE) in human skin samples?

Asked by: Preethi
Thank you for your question. We recommend a starting dilution of 1:50 (with a range of 1:50-1:500) for IHC(P) using this mouse monoclonal antibody, Rab 27a (E-8): sc-74586. A link to our recommended protocol is provided below for additional details. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-05-17

What is the recommended secondary when using this Rab27a mouse monoclonal antibody?

Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-18
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Rated 4 out of 5 by from Good antibody! I have tried it for the CLL cell line, it worked good.
Date published: 2018-04-06
Rated 5 out of 5 by from great ab Seems to react well with mouse Rab27a by western.. Pretty much as advertised... very happy with it.
Date published: 2017-12-06
Rated 5 out of 5 by from Wow !! Superb product Very clear western blot data;strong signal with low background; superb IHC staining !!
Date published: 2017-02-01
Rated 5 out of 5 by from Excellent! This antibody works great in western blot analysis of Rab 27a expression in C32 whole cell lysate.
Date published: 2017-01-10
Rated 5 out of 5 by from Strong detection seen with HL Strong detection seen with HL-60, C32 and SK-MEL-28 whole cell lysates -SCBT QC
Date published: 2015-02-21
Rated 5 out of 5 by from Good staining seen in IHC with paraffin Good staining seen in IHC with paraffin-embedded human stomach tissue -SCBT QC
Date published: 2014-06-09
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