Anti-Rab 27a Antibody (E-8) is a mouse monoclonal IgG2b κ Rab 27a antibody, cited in 10 publications, provided at 200 µg/ml
raised against amino acids 162-221 mapping at the C-terminus of Rab 27a of human origin
Anti-Rab 27a Antibody (E-8) is recommended for detection of Rab 27a of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
Anti-Rab 27a Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Rab 27a (E-8): sc-74586.
m-IgGκ BP-HRP is the preferred secondary detection reagent for Rab 27a Antibody (E-8) for WB and IHC(P) applications. This reagent is now offered in a bundle with Rab 27a Antibody (E-8) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Rab 27a Antibody (E-8) is a high quality monoclonal Rab 27a antibody (also designated Ras-related protein Rab-27A antibody, Rab family small GTPase 27 antibody, RAB27 antibody, Griscelli syndrome type 2 antibody, or GS2 antibody) suitable for the detection of the Rab 27a protein of mouse, rat and human origin. Rab 27a Antibody (E-8) is available as both the non-conjugated anti-Rab 27a antibody form, as well as multiple conjugated forms of anti-Rab 27a antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The Rab family of low molecular weight GTPases are critical regulators of vesicular transport. Rab proteins cycle between an active GTP-bound state, which recruits specific effector proteins, and an inactive GDP-bound state. Two members of this family, Rab27a and Rab27b, have overlapping functions, but differ in tissue specificity. Rab27a is widely expressed with significant expression in pancreatic islets and pituitary tissue, and low expression in brain. Rab27b is also expressed in pituitary tissue, but is more significantly expressed in brain and spleen. Rab27a regulates diverse processes involving lysosome-related organelles, including melanosome motility in melanocytes and lytic granule release in cytotoxic T lymphocytes. Mutations in the Rab27a gene result in Griscelli syndrome (GS) or the corresponding mouse model ashen, a rare autosomal recessive disorder characterized by hypopigmentation, prolonged bleeding times, and platelet storage pool deficiency (2,4). In GS, Rab27a is not available to mediate the recruitment of melanosomes via the actin motor, myosin Va. The human Rab27b gene maps to chromosome 18q21.1, and encodes a protein that is involved in pituitary hormone secretion. Rab27b may be functionally redundant to Rab27a, as it can rescue Rab27a mutants.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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