Anti-Rab 25 Antibody (E-7) recommended for detection of Rab 25 of human origin by WB, IP, IF and ELISA

Anti-Rab 25 Antibody (E-7): sc-166143

Anti-Rab 25 Antibody (E-7) is rated 5.0 out of 5 by 2.
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Datasheets

    • Rab 25 Antibody (E-7) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 163-213 mapping at the C-terminus of Rab 25 of human origin
    • recommended for detection of Rab 25 of human origin by WB, IP, IF and ELISA
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Rab 25 Antibody (E-7) for WB applications. These reagents are now offered in bundles with Rab 25 Antibody (E-7) (see ordering information below).
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What is the recommended starting dilution for Western blot for sc-166143: Rab 25 (E-7) monoclonal antibody?

Asked by: TinTin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Signal via western blot analysis of Rab 25 Signal via western blot analysis of Rab 25 expression in non-transfected: sc-117752 and human Rab 25 transfected: sc-114009 293T whole cell lysates. -SCBT QC
Date published: 2015-04-24
Rated 5 out of 5 by from Western blot publication using human AGS Western blot publication using human AGS gastric cancer cell samples -SCBT Publication Review
Date published: 2014-10-31
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