Anti-Rab 14 Antibody (D-5) recommended for detection of Rab 14 of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA

Anti-Rab 14 Antibody (D-5): sc-271401

Anti-Rab 14 Antibody (D-5) is rated 5.0 out of 5 by 3.
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Datasheets

    • Rab 14 Antibody (D-5) is a mouse monoclonal IgG1, cited in 5 publications, provided at 200 µg/ml
    • raised against amino acids 161-215 mapping at the C-terminus of Rab 14 of human origin
    • Rab 14 Antibody (D-5) is recommended for detection of Rab 14 of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
    • Anti-Rab 14 Antibody (D-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Rab 14 (D-5): sc-271401.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Rab 14 Antibody (D-5) for WB and IHC(P) applications. These reagents are now offered in bundles with Rab 14 Antibody (D-5) (see ordering information below).
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Are there any issues with using sc-271401: Rab 14 (D-5) mouse monoclonal primary antibody on mouse samples?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-271401 conjugates.
Answered by: Technical Support
Date published: 2017-03-07

What would the appropriate isotype control antibody be for this antibody? Mouse IgG1?

Asked by: MichaelC
Thank you for your question. The proper isotype control for our Rab 14 antibody sc-271401 would be a normal mouse IgG1, such as sc-3877 (https://www.scbt.com/scbt/product/normal-mouse-igg1).
Answered by: Technical Service
Date published: 2016-11-30
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Rated 5 out of 5 by from Powerful signal We received this antibody as a free trial sample a month ago. Due to the fact that it is a little amount we only assayed one dilution on western blotting (1/500). Signal was great and the band, well defined. We are really happy with the quality of this antibody and we aim to test it on other techniques( IF and FC).
Date published: 2017-10-20
Rated 5 out of 5 by from Excellent! This antibody is great in western blot analysis of Rab 14 expression in LADMAC and Hep G2 whole cell lysates.
Date published: 2017-01-26
Rated 5 out of 5 by from Western Blot of LADMAC and Hep G2 whole cell Western Blot of LADMAC and Hep G2 whole cell lysates showed very strong bands -SCBT QC
Date published: 2014-09-17
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