Anti-Rab 11A Antibody (A-6) recommended for detection of Rab 11A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Rab 11A Antibody (A-6): sc-166912

Anti-Rab 11A Antibody (A-6) is rated 4.3 out of 5 by 12.
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Datasheets

    • Anti-Rab 11A Antibody (A-6) is a mouse monoclonal IgG2b κ Rab 11A antibody, cited in 14 publications, provided at 200 µg/ml
    • raised against amino acids 130-216 of Rab 11A of human origin
    • Anti-Rab 11A Antibody (A-6) is recommended for detection of Rab 11A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
    • Anti-Rab 11A Antibody (A-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Rab 11A (A-6): sc-166912.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Rab 11A Antibody (A-6). This work is in progress.
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Does Rab 11A Antibody (A-6): sc-166912 only recognize Rab11A? Or can the antibody also bind to Rab11B?

Asked by: ila123
Thank you for your question. This monoclonal antibody is raised against an antigen that is specific for RAB 11A and does not cross react with Rab 11B. However, we have not tested the antibody for cross reactivity with Rab 11B specifically.
Answered by: Technical Support
Date published: 2019-02-23

I have been trying to do immuno-fluorescence with the antibody using a standard Immunostaing protocol. I have tried dilution of 1:10 also, but not got any staining. The antibody gives a good band in western blot . Please kindly suggest.

Asked by: RUCHIR
Thank you for your question. We are happy to help you in troubleshooting this antibody. Please contact our Technical Service Department for support. Please call our Asian Support office toll-free at 800-820-8626, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2018-01-31

What is the recommended fixation method for IF with Rab 11A (A-6): sc-166912 monoclonal antibody?

Asked by: jenniferc
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support 8
Date published: 2017-02-03
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Rated 3 out of 5 by from Staining works I got a sample and tested it. The staining which I used 1:100 dilution is working on MEF cells.
Date published: 2019-05-23
Rated 4 out of 5 by from IF観察 数カ月前に提供して頂きました。動物培養細胞の顆粒マ-カ-を検索していますが、陽性マ-カ-として利用できそうなのでさらに別の陽性コントロ-ルを加え、追跡中です。
Date published: 2018-06-15
Rated 3 out of 5 by from Not good We received a sample and used it in human colon carcinoma cells. We could not detect a band of right size in western blot.
Date published: 2018-04-03
Rated 5 out of 5 by from Worked for WB Received as free sample. Good detection, low background. 1/1000. Blocked in 2% BSA
Date published: 2018-03-07
Rated 5 out of 5 by from great antibody for both WB and IF We received a sample and used it in human colon carcinoma cells. We saw cytoplasmic dots in IF and a clear band of right size in western blot.
Date published: 2017-10-25
Rated 5 out of 5 by from works great on mouse cell line cell extract. Highl works great on mouse cell line cell extract. Highly recommended.
Date published: 2017-08-01
Rated 1 out of 5 by from Apparently it does not work Blotting on primary rat cortices (cell culture), 20 ug protein per lane, 1:50 dilution, control tubulin band excellent, Rab11a band nonexistent.
Date published: 2017-06-03
Rated 5 out of 5 by from good staining Good data obtained by IF using human kidney tissue.
Date published: 2017-05-27
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