Anti-PTP1B Antibody (B-9) recommended for detection of PTP1B of human origin by WB, IP, IF and ELISA

Anti-PTP1B Antibody (B-9): sc-133115

Anti-PTP1B Antibody (B-9) is rated 5.0 out of 5 by 2.
  • y_2022, m_1, d_15, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_133115, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 120ms
  • REVIEWS, PRODUCT
loading
5
2
4
0
3
0
2
0
1
0

Datasheets

    • PTP1B Antibody (B-9) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 301-435 mapping at the C-terminus of PTP1B (protein tyrosine phosphatase) of human origin
    • recommended for detection of PTP1B of human origin by WB, IP, IF and ELISA
    • See PTP1B (D-4): sc-133259 for PTP1B antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for PTP1B Antibody (B-9) for WB applications. These reagents are now offered in bundles with PTP1B Antibody (B-9) (see ordering information below).
loading

What blocking solution should I use with PTP1B (B-9): sc-133115 monoclonal antibody for Western blot?

Asked by: Germaine
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-27
  • y_2022, m_1, d_15, h_20CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_133115, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 94ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Nicely worked for WB and IF in epithelial cells This antibody worked perfectly in WB and IF in non-ransfected human bronchial epithelial cell lines.
Date published: 2017-09-27
Rated 5 out of 5 by from Satisfactory Western blot data of PTP1B expression Satisfactory Western blot data of PTP1B expression in non-transfected and human PTP1B transfected 293 whole cell lysates. -SCBT QC
Date published: 2014-10-28
  • y_2022, m_1, d_15, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_133115, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 8ms
  • REVIEWS, PRODUCT