Thank you for your question. You can find our recommended protocol for Immunoprecipitation/Western Blotting by following this link: https://www.scbt.com/scbt/de/resources/protocols/immunoprecipitation-western-blots
Protein G PLUS-Agarose is used in the preclearing step of the cell lysate together with a control antibody, which removes proteins from the lysate that would cause unspecific results in later steps.
If you are not using a primary antibody agarose conjugate you will also have to use Protein G PLUS-Agarose when incubating your cell lysate with the specific antibody.
For further assistance, please get in touch with our technical service.
Date published: 2019-05-06