Protein G PLUS-Agarose immunoprecipitation reagent to reduce non-specific immunoglobulin binding

Protein G PLUS-Agarose: sc-2002

Protein G PLUS-Agarose is rated 5.0 out of 5 by 23.
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Datasheets

    • provided as 0.5 ml agarose/2.0 ml; 100 reactions
    • suitable for use at 20 µl per immunoprecipitation reaction
    • pre-treated to reduce non-specific immunoglobulin binding
    • linked to Sepharose CL-6B
    • binding capacity is 10 mg of antibody per 1 ml of packed beads, settled not spun
    • strong binding to mouse IgG1 , mouse IgG2a , IgG2b and IgG3 , rat IgG2a and IgG2c , human IgG1 , IgG2 , IgG3 and IgG4 , rabbit, bovine and ovine IgG
    • weak to medium binding to rat IgG1 , rat IgG2b , human IgM, IgA and IgE, porcine, canine, feline and guinea pig IgG
    • pre-washed and ready to use
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Hello there, the provided protocol for IP mentioned elution is achieved via "boil". So in the "boil" condition, whether or not the agarose beads would melt?

Asked by: Jacksonnn
Thank you for your question. The agarose beads will not melt under these conditions. They are stable under this high temperature. We recommend to "boil" at 95C for 5 minutes. This is the recommended method if you are using SDS buffer for elution. High temperature denatures protein and promotes denaturation by SDS.
Answered by: Technical Support
Date published: 2020-05-18

Hi there, I have a paper which mention protein G-Sepharose from Santa Cruz Biothechnology (it does not say the catalog number/ref number). When I googled it I got this PROTEIN G PLUS-AGAROSE sc2002. Could you confirm whether it is a right product for IP?

Asked by: Zsudy
Thank you for your question. Yes, sc-2002 is designed to be used for IP applications, so it is recommended for IP use.
Answered by: Tech Service
Date published: 2019-10-28

Which step should I use sc-2002 at, during western blot?

Asked by: yeji
Thank you for your question. You can find our recommended protocol for Immunoprecipitation/Western Blotting by following this link: https://www.scbt.com/scbt/de/resources/protocols/immunoprecipitation-western-blots Protein G PLUS-Agarose is used in the preclearing step of the cell lysate together with a control antibody, which removes proteins from the lysate that would cause unspecific results in later steps. If you are not using a primary antibody agarose conjugate you will also have to use Protein G PLUS-Agarose when incubating your cell lysate with the specific antibody. For further assistance, please get in touch with our technical service.
Answered by: Technical Support Europe
Date published: 2019-05-06
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Rated 5 out of 5 by from It works I bought this item about a month ago. I tried it and it seems to be working.
Date published: 2020-07-24
Rated 5 out of 5 by from Good deal It is less expensive and works for Immunoprecipitation with mouse and rabbit Ig.
Date published: 2019-08-24
Rated 5 out of 5 by from Works Great! We continue to buy this over and over again, and we're so happy with the results!
Date published: 2019-04-26
Rated 5 out of 5 by from Works well We have already bought it twice and works well. A+
Date published: 2018-03-20
Rated 5 out of 5 by from Great for immunoprecipitation Excellent for immunoprecipitation with mouse antibodies. We have purchased this four times already, and it worked everytime with just 50 ul per sample.
Date published: 2018-02-02
Rated 4 out of 5 by from Bueno Funciona muy bien en inmunoprecipitaciones utilizando extractos celulares totales
Date published: 2018-01-08
Rated 5 out of 5 by from excellent product for IP I used this product many times, it worked very well.
Date published: 2017-02-04
Rated 5 out of 5 by from excellent quality and reasonable price It works very well for IP .It considering we can't find a good product anywhere else. Thanks
Date published: 2017-01-22
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