Anti-PRMT1 Antibody (B-2) recommended for detection of PRMT1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-PRMT1 Antibody (B-2): sc-166963

Anti-PRMT1 Antibody (B-2) is rated 4.9 out of 5 by 10.
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Datasheets

    • Anti-PRMT1 Antibody (B-2) is a mouse monoclonal IgG1 κ PRMT1 antibody, cited in 14 publications, provided at 200 µg/ml
    • raised against amino acids 166-300 mapping within an internal region of PRMT1 of human origin
    • Anti-PRMT1 Antibody (B-2) is recommended for detection of PRMT1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-PRMT1 Antibody (B-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PRMT1 (B-2): sc-166963.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for PRMT1 Antibody (B-2) for WB and IHC(P) applications. These reagents are now offered in bundles with PRMT1 Antibody (B-2) (see ordering information below).
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I want to use this antibody to make a experiment of immunoprecipitation in the mouse preadipocytes,can you ensure you product can satisfy my requirement ? Or maybe I need to apply for a trial product.

Asked by: Anonymous
Thanks for your questions. Anti-PRMT1 Antibody (B-2) is recommended for detection of PRMT1 of mouse origin by IP. 感谢您的问题。Anti-PRMT1 抗体 (B-2)是推荐免疫沉淀方法检测小鼠来源样本的,该产品不质保免疫共沉淀。
Answered by: Technical Support 9
Date published: 2020-06-12

I am using PRMT1 (B-2): sc-166963 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from high-active Strong bands were observed in human tissues by immunoblotting, and it was very effective for WB.
Date published: 2017-10-26
Rated 5 out of 5 by from good performance I usually use this PRMT1 anitbody .This PRMT1 antibody worked very well for WB, it be diluted 1:1000,we got clear and strong signal at 42kda
Date published: 2017-09-02
Rated 5 out of 5 by from Excellent antibody a free sample, but a good result !! I will continue to support SCBT
Date published: 2017-08-15
Rated 4 out of 5 by from this antibody also can be used mouse ES cells this antibody also can be used mouse ES cells and can be detected about 35KDa.
Date published: 2017-06-27
Rated 5 out of 5 by from good result. This was used for IP detection in AGS, when diluted 1:1000 is a good signal.
Date published: 2017-06-21
Rated 5 out of 5 by from specific signal I am glad to buy this antibody. I used it to do western blot .It have signal in Hela cell line.
Date published: 2017-05-31
Rated 5 out of 5 by from Good band robust bands seen in WB using Jurkat and K-562 nuclear extracts.
Date published: 2017-02-24
Rated 5 out of 5 by from Good specificity in WB in COLO 320DM Good specificity in WB in COLO 320DM, HeLa, IMR-32 and WI 38 whole cell lysates. -SCBT QC
Date published: 2015-06-19
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