Anti-PREP-1 Antibody (B-2) recommended for detection of PREP-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-PREP-1 Antibody (B-2): sc-25282

Anti-PREP-1 Antibody (B-2) is rated 5.0 out of 5 by 2.
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Datasheets

    • Anti-PREP-1 Antibody (B-2) is a mouse monoclonal IgG1 κ PREP-1 antibody, cited in 9 publications, provided at 200 µg/ml
    • Anti-PREP-1 Antibody (B-2) is recommended for detection of PREP-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-PREP-1 Antibody (B-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-25282 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PREP-1 (B-2): sc-25282.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PREP-1 Antibody (B-2) for WB and IHC(P) applications. These reagents are now offered in bundles with PREP-1 Antibody (B-2) (see ordering information below).
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Dear Sir, I have 2 quesions: 1. Does this antibody work well for chip(PREP-1 Antibody (B-2) sc-25282)? 2. Does this antibody still work for chip and immunohistochemical experiment after left at room temperature overnight? Thank you!

Asked by: zhidong
Thank you for your question. PREP-1 Antibody (B-2): sc-25282 is recommended for detection of PREP-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA, so you can perform immunohistochemistry with this antibody using the standard protocol provided on our website. A TransCruz reagent for Gel Supershift and ChIP applications with higher concentration and an optimized buffer is also available for this antibody: sc-25282 X. You can also take a look at publication PMID 30337366, in which the antibody has been used for ChIP.
Answered by: Tech Support Europe
Date published: 2019-06-06

When should the TransCruz reagent sc-25282 X be used instead of the standard form sc-25282?

Asked by: Trav11
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Produced great immunoperoxidase nuclear staining Produced great immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human cervical cancer tissue. -SCBT QC
Date published: 2015-05-02
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