Anti-PR3 Antibody (D-1) recommended for detection of precursor and mature forms of PR3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-PR3 Antibody (D-1): sc-74534

Anti-PR3 Antibody (D-1) is rated 4.7 out of 5 by 3.
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Datasheets

    • Anti-PR3 Antibody (D-1) is a mouse monoclonal IgG1 PR3 antibody, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 89-148 mapping within an internal region of PR3 of human origin
    • Anti-PR3 Antibody (D-1) is recommended for detection of precursor and mature forms of PR3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-PR3 Antibody (D-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PR3 (D-1): sc-74534.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for PR3 Antibody (D-1) for WB and IHC(P) applications. These reagents are now offered in bundles with PR3 Antibody (D-1) (see ordering information below).
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What disorders does this test check for 

Asked by: MicheleC
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Anonymous
Date published: 2022-01-28

Can PR3 (D-1): sc-74534 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, PR3 (D-1): sc-74534 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good antibody Detects single band around 29 kD on 20 ug of THP-1 lystate following overnight incubation at 1:200 dilution.
Date published: 2017-09-21
Rated 5 out of 5 by from Exceptional immunoperoxidase cytoplasmic Exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human spleen tissue. -SCBT QC
Date published: 2015-02-06
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