Anti-PPAR gamma 2 Antibody (A-1) recommended for detection of PPARγ2 of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-PPARγ2 Antibody (A-1): sc-166731

Anti-PPARγ2 Antibody (A-1) is rated 4.6 out of 5 by 5.
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Datasheets

    • Anti-PPARγ2 Antibody (A-1) is a mouse monoclonal IgG2a κ PPARγ2 antibody, cited in 12 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 2-20 at the N-terminus of PPARγ2 of human origin
    • PPAR gamma 2 Antibody (A-1) is recommended for detection of PPARγ2 of human origin by WB, IP, IF, IHC(P) and ELISA; non cross-reactive with PPARγ1
    • TransCruz reagent for ChIP application (sc-166731 X, 200 µg/0.1 ml)
    • Anti-PPAR gamma 2 Antibody (A-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PPARγ2 (A-1): sc-166731.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for PPARγ2 Antibody (A-1) for WB and IHC(P) applications. These reagents are now offered in bundles with PPARγ2 Antibody (A-1) (see ordering information below).

What application is the blocking peptide sc-166731 P appropriate for?

Asked by: Dr Ninau Qelp
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from 条带专一 用于检测3T3L1脂肪前细胞系中脂肪诱导分化后PPARG2的表达情况,得到符合目的大小条带,符合实验预期。
Date published: 2017-07-14
Rated 5 out of 5 by from Produced publishable WB data on human This well characterized PPar gamma 2 antibody has several references in the literature for the use in WB on human samples
Date published: 2017-01-26
Rated 5 out of 5 by from Works well for IF applications Works well for IF applications, positive in HepG2. -SCBT QC
Date published: 2015-01-20
Rated 5 out of 5 by from Great staining with paraffin Great staining with paraffin-embedded human gall bladder -SCBT QC
Date published: 2015-01-18
Rated 4 out of 5 by from Worked with transfected lysate by WB Worked with transfected lysate by WB -SCBT QC
Date published: 2014-04-23
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