Anti-PPARγ Antibody (E-8) is a mouse monoclonal IgG1 κ PPARγ antibody, cited in 991 publications, provided at 200 µg/ml
specific for an epitope mapping between amino acids 486-505 at the C-terminus of PPARγ of human origin (identical to corresponding mouse sequence)
Anti-PPAR gamma Antibody (E-8) is recommended for detection of PPARγ1 and PPARγ2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
PPAR gamma Antibody (E-8) is available conjugated to agarose for IP; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to biotin for WB, IHC(P) and ELISA; and to either TRITC or Alexa Fluor® 405 for IF, IHC(P) and FCM
TransCruz reagent for Gel Supershift and ChIP applications (sc-7273 X, 200 µg/0.1 ml)
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PPARγ (E-8): sc-7273.
m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for PPARγ Antibody (E-8) for WB and IHC(P) applications. These reagents are now offered in bundles with PPARγ Antibody (E-8) (see ordering information below).
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Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
PPARγ Antibody (E-8) is a high quality monoclonal PPAR gamma antibody (also designated PPARG antibody) suitable for the detection of the PPAR gamma protein of mouse, rat and human origin. PPARγ Antibody (E-8) is available as both the non-conjugated anti-PPAR gamma antibody form, as well as multiple conjugated forms of anti-PPAR gamma antibody, including agarose, PE, FITC and multiple Alexa Fluor® conjugates. PPARs are members of the nuclear hormone receptor subfamily of transcription factors. PPARs form heterodimers with retinoid X receptors (RXRs). These heterodimers regulate transcription of genes involved in insulin action, adipocyte differentiation, lipid metabolism and inflammation. PPARγ is implicated in numerous diseases including obesity, diabetes, atherosclerosis and cancer, making the PPARγ antibody an important research tool. PPARγ activators include prostanoids, fatty acids, thiazolidinediones and N-(2-benzoylphenyl) tyrosine analogues. A key component in adipocyte differentiation and fat-specific gene expression, PPARγ may modulate macrophage functions such as proinflammatory activities, and stimulate oxidized low-density lipoprotein (x-LDL) uptake. A Pro12Ala polymorphism of the PPARγ2 gene has been reported to reduce transactivation activity in vitro. This substitution may affect the immune response to ox-LDL and be associated with type 2 diabetes. In addition, the Pro12Ala variant of the PPARγ2 gene maybe correlated with abdominal obesity in type 2 diabetes. The PPARγ Antibody (E-8) is recommended for detection of both PPARγ1 and PPARγ2 gene products.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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