Anti-PPAR gamma Antibody (B-5) recommended for detection of PPARγ1 and PPARγ2 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-PPARγ Antibody (B-5): sc-271392

Anti-PPARγ Antibody (B-5) is rated 4.4 out of 5 by 11.
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Datasheets

    • PPARγ Antibody (B-5) is a mouse monoclonal IgG1 λ, cited in 46 publications, provided at 200 µg/ml
    • raised against amino acids 8-106 of PPARγ of human origin
    • Anti-PPAR gamma Antibody (B-5) is recommended for detection of PPARγ1 and PPARγ2 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-PPAR gamma Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-271392 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PPARγ (B-5): sc-271392.
    • m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for PPARγ Antibody (B-5) for WB applications. This reagent is now offered in a bundle with PPARγ Antibody (B-5) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Is this antibody available as "unconjugated?"

Asked by: 23Cae
Thank you for your question. Yes, the standard format for this and all antibodies is unconjugated. Please reference PPARγ Antibody (B-5): sc-271392. This antibody is also available conjugated. Please refer to the product block above for which conjugated forms are available.
Answered by: Technical Support
Date published: 2019-01-26

do you have peptide for PPARr B5 sc-271392?

Asked by: Haimei
Thank you for your question. This mouse monoclonal antibody is not raised against a peptide, it is raised against a recombinant protein corresponding to amino acids 8-106 of PPARγ of human origin. No blocking peptide is available.
Answered by: Technical Support
Date published: 2019-01-26

I am Vishva from Ohio University. I would like to try two of your antibodies (SC-271392 and SC-6247). Would it be possible for you to send me the free sample for the trial. If it works, I can then buy these products.

Asked by: Vishva
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: Technical Support
Date published: 2018-12-14

Does PPARγ Antibody (B-5): sc-271392 react with avian samples?

Asked by: sweetums
Thank you for your question. For additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or by using the Live Chat function on our website and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Technical Support 13
Date published: 2017-01-20

When should the TransCruz reagent sc-271392 X be used instead of the standard form sc-271392? What is the difference between the two forms?

Asked by: sweetums
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration. The TransCruz reagent is provided as 10x more concentrated than the regular form: sc-271392 X, 200 µg/0.1 ml.
Answered by: Technical Support 1
Date published: 2017-01-16
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Rated 5 out of 5 by from 功能很出色 已经购买过santacruz的一抗,santacruz的抗体效果还是不错的,还有小包装和试用装。很赞!
Date published: 2020-05-20
Rated 1 out of 5 by from didn't work it didn't work but we've only tried once. dillution 1/500
Date published: 2019-04-01
Rated 5 out of 5 by from PPARgamma in rat livers Received this antibody as a sample. Was able to detect the protein in rat livers. However, the antibody needs to be incubated overnight in blocking buffer.
Date published: 2018-07-11
Rated 5 out of 5 by from The antibody works well. I bought the antibody a month ago, and I am happy that It did work very well.
Date published: 2017-11-26
Rated 5 out of 5 by from Strong western Very nice western data; strong signal with low background
Date published: 2017-01-26
Rated 5 out of 5 by from No Non-specific Bands Very clean blots seen in WB, single strong target band with no additional signal.
Date published: 2017-01-17
Rated 4 out of 5 by from Good specificity in WB at 1:200 dilution Good specificity in WB at 1:200 dilution in human prostate cancer cell lines. -SCBT Publication Review
Date published: 2015-03-19
Rated 5 out of 5 by from Good antibody for IHC at 1:200 dilution in Good antibody for IHC at 1:200 dilution in human aortic wall segments. -SCBT Publication Review
Date published: 2015-03-13
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