Anti-PPAR gamma Antibody (8D1H8H4) recommended for detection of PPARγ of mouse, rat and human origin by WB, IF and IHC(P)

Anti-PPARγ Antibody (8D1H8H4): sc-81152

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Anti-PPARγ Antibody (8D1H8H4) is rated 4.0 out of 5 by 1.
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Datasheets

    • PPARγ Antibody (8D1H8H4) is a mouse monoclonal IgG1, cited in 9 publications, provided at 200 µg/ml
    • raised against a recombinant protein corresponding to amino acids 77-272 of PPARγ of human origin
    • recommended for detection of PPARγ of mouse, rat and human origin by WB, IF and IHC(P)
    • See PPARγ (E-8): sc-7273 for PPARγ antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for PPARγ Antibody (8D1H8H4). This work is in progress.
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we used this antibody at 1:500 and companied with secondary antibody (Cell Signalling #7076s), all we see on blot was IgG 50kd and 25kd. so we tested only secondary antibody (without primary PPARr 81152), there were strong IgG both 25kd and 50 on blot.

Asked by: Haimei
Thank you for your question. Are you using an IP'd lysate? It sounds like you are detecting the heavy and light chains of a denatured Ig. Please contact Technical Service for further assistance. We can be reached by phone, 1800-457-3801 option 2, by email [email protected] or by live chat on our website, www.scbt.com.
Answered by: Santa Cruz TS
Date published: 2021-07-10

I need to use an antibody to detect the protein in samples of monkey origin. Do you know if sc-81152: PPARγ (8D1H8H4) mouse monoclonal antibody will work?

Asked by: cjMara
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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