Anti-PPAR beta Antibody (F-7) recommended for detection of PPARβ of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-PPARβ Antibody (F-7): sc-74440

Anti-PPARβ Antibody (F-7) is rated 4.4 out of 5 by 7.
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Datasheets

    • PPARβ Antibody (F-7) is a mouse monoclonal IgG1 κ, cited in 8 publications, provided at 200 µg/ml
    • raised against amino acids 2-75 of PPARβ of human origin
    • recommended for detection of PPARβ of human origin by WB, IP, IF, IHC(P) and ELISA
    • See PPARβ (F-10): sc-74517 for PPARβ antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for PPARβ Antibody (F-7) for WB and IHC(P) applications. These reagents are now offered in bundles with PPARβ Antibody (F-7) (see ordering information below).

I am using PPARβ (F-7): sc-74440 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Professor Griffin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Clean Clean, crisp bands observed by Western Blot, showing an increase in human-derived cells (HCECs) PPAR expression following treatment with cytokines. -SCBT Publication Review
Date published: 2015-04-30
Rated 4 out of 5 by from By immunohistochemistry (formalin fixed By immunohistochemistry (formalin fixed, paraffin embedded), nuclear staining was observed in trophoblastic cells of human placenta tissue. -SCBT QC
Date published: 2015-04-02
Rated 4 out of 5 by from Quiescent HPASMCs were treated with PDGF Quiescent HPASMCs were treated with PDGF for various amounts of time, up to 72 hours, then extracted for analysis by Western Blot. Using the sc-74440 antibody, an increase in PPAR expression was observed. -SCBT Publication Review
Date published: 2015-04-01
Rated 5 out of 5 by from Two strong bands at the correct molecular Two strong bands at the correct molecular weights (Isoform 1 & 2) of PPAR observed by Western Blot for Jurkat whole cell lysate. -SCBT QC
Date published: 2015-03-27
Rated 5 out of 5 by from Beautiful staining by immunofluorescence Beautiful staining by immunofluorescence demonstrating the expression of PPAR in rat corneal epithelia during wound healing. Good staining as well on samples after treatment with GW501516, a PPAR agonist. -SCBT Publication Review
Date published: 2015-03-20
Rated 3 out of 5 by from Analysis by Western blot Analysis by Western blot, it was found that HPASMCs have higher expression levels of PPAR than HPAECs. Bands for both cell lines were observed to be faint. -SCBT Publication Review
Date published: 2015-03-10
Rated 5 out of 5 by from Crisp Crisp, clean band observed by Western Blot, using human recombinant PPAR fusion protein. -SCBT QC
Date published: 2014-07-18
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