Anti-PPAR beta Antibody (F-10) recommended for detection of PPARβ of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-PPARβ Antibody (F-10): sc-74517

Anti-PPARβ Antibody (F-10) is rated 4.6 out of 5 by 21.
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Datasheets

    • Anti-PPARβ Antibody (F-10) is a mouse monoclonal IgG1 κ PPARβ antibody, cited in 39 publications, provided at 200 µg/ml
    • raised against amino acids 2-75 of PPARβ of mouse origin
    • Anti-PPAR beta Antibody (F-10) is recommended for detection of PPARβ of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-PPAR beta Antibody (F-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-74517 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PPARβ (F-10): sc-74517.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for PPARβ Antibody (F-10) for WB applications. These reagents are now offered in bundles with PPARβ Antibody (F-10) (see ordering information below).
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I need to use an antibody to detect the protein in samples of avian origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 13
Date published: 2017-01-12

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with PPARβ (F-10): sc-74517?

Asked by: jerojero
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-12
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Rated 1 out of 5 by from da ritestare non sono convinta. Mi riprometto di ritestare l'anticorpo PPARβ cambiando condizioni e tipi cellulari
Date published: 2020-11-18
Rated 2 out of 5 by from good antibody we tested this antibody in wb in dilution 1/250 in Hela cells. the bands were detected were faint.
Date published: 2019-04-01
Rated 5 out of 5 by from Great antibody I have used this antibody for protein extracts from breast cancer cell lines and it works really well
Date published: 2018-07-31
Rated 5 out of 5 by from Great! I used this antobody in different mouse breast cancer cell lines and got clear bands with the expected molecular weight.
Date published: 2017-12-12
Rated 5 out of 5 by from The antibody works well. I bought the antibody a month ago, and I am happy that it did work very well.
Date published: 2017-11-26
Rated 5 out of 5 by from The effect is very good. PPARβ Antibody is used to detect immunofluorescence, the signal specificity is very strong, the signal is also strong.
Date published: 2017-10-25
Rated 5 out of 5 by from This antibody works well PPARβ Antibody are amazing. It proves helpful and efficient and give us a pretty impression in whole cell lysates, the results was satisfactory.
Date published: 2017-10-22
Rated 5 out of 5 by from Exciting result PPARβ Antibody is our ideal choice to do drugtest because it has good quality in Western blot and can improves the detecting sensitivity.
Date published: 2017-10-18
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