Anti-PPAR alpha Antibody (467D1a) recommended for detection of PPARα of human origin by WB and IP

Anti-PPARα Antibody (467D1a): sc-130640

Anti-PPARα Antibody (467D1a) is rated 4.5 out of 5 by 2.
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Datasheets

    • PPARα Antibody (467D1a) is a mouse monoclonal IgG1, cited in 10 publications, provided at 100 µg/ml
    • raised against a recombinant protein corresponding an internal region of PPARα of human origin
    • recommended for detection of PPARα of human origin by WB and IP
    • See PPARα (H-2): sc-398394 for PPARα antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for PPARα Antibody (467D1a). This work is in progress.
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I am using PPARα (467D1a): sc-130640 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Professor Griffin
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Worked well in WB with recombinant PPAR alpha Worked well in WB with recombinant PPAR alpha fusion protein. -SCBT QC
Date published: 2015-03-28
Rated 5 out of 5 by from Strong band in WB in human HepG2 cells Strong band in WB in human HepG2 cells. -SCBT Publication Review
Date published: 2014-10-30
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