Positive cofactor 4 Antibody (H-12)recommended for detection of Positive cofactor 4 of human origin by WB, IP, IF, IHC(P) and ELISA

Positive cofactor 4 Antibody (H-12): sc-166280

Positive cofactor 4 Antibody (H-12) is rated 5.0 out of 5 by 2.
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Datasheets

    • Positive cofactor 4 Antibody (H-12) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 14-127 mapping at the C-terminus of Positive cofactor 4 of human origin
    • Positive cofactor 4 Antibody (H-12) is recommended for detection of Positive cofactor 4 of human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine and porcine
    • Positive cofactor 4 Antibody (H-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-166280 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Positive cofactor 4 (H-12): sc-166280.
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When should the TransCruz reagent sc-166280 X be used instead of the standard form sc-166280?

Asked by: Germaine
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Western Blot I got this antibody as a free 10 ul sample, and used it in Western Blot of HeLa cell lines
Date published: 2017-08-26
Rated 5 out of 5 by from Produced positive Western Blot data of Positive Produced positive Western Blot data of Positive cofactor 4 expression in non-transfected 293, human Positive cofactor 4 transfected 293 and Jurkat whole cell lysates. -SCBT QC
Date published: 2013-07-16
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