PON2 Antibody (C-5)recommended for detection of PON2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

PON2 Antibody (C-5): sc-374158

PON2 Antibody (C-5) is rated 5.0 out of 5 by 5.
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Datasheets

    • PON2 Antibody (C-5) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 61-113 mapping within an internal region of PON2 of human origin
    • PON2 Antibody (C-5) is recommended for detection of PON2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • PON2 Antibody (C-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PON2 (C-5): sc-374158.
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Can PON2 (C-5): sc-374158 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Germaine
Thank you for your question. Yes, PON2 (C-5): sc-374158 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Nice staining and strong western blotting bands This antibody worked really well for both immunostaining and western blot
Date published: 2018-03-13
Rated 5 out of 5 by from Quite strong band! 1:200 diluted for wb of rat protein,the band is strong and special!
Date published: 2018-01-24
Rated 5 out of 5 by from Robust signal in WB in HeLa and HepG2 whole Robust signal in WB in HeLa and HepG2 whole cell lysates. -SCBT QC
Date published: 2015-05-23
Rated 5 out of 5 by from Strong band in WB in human transfected 293T Strong band in WB in human transfected 293T, HeLa, Hep G2, A549 and U-251-MG whole cell lysates. -SCBT QC
Date published: 2015-04-13
Rated 5 out of 5 by from Great immunoperoxidase cytoplasmic staining Great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human duodenum tissue. -SCBT QC
Date published: 2015-02-19
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