Anti-PON1 Antibody (17A12) is a mouse monoclonal IgG1 κ PON1 antibody, cited in 2 publications, provided at 100 µg/ml
raised against His tagged recombinant PON1 of human origin
recommended for detection of PON1 of mouse, rat and human origin by WB and IP
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PON1 (17A12): sc-59646.
m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) is the preferred secondary detection reagent for PON1 Antibody (17A12) for WB applications. This reagent is now offered in a bundle with PON1 Antibody (17A12) (see ordering information below).
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
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Labware ships FedEx Ground free of charge to the contiguous US
PON1 Antibody (17A12) is a high quality monoclonal PON1 antibody (also designated PON1 antibody) suitable for the detection of the PON1 protein of mouse, rat and human origin. PON1 Antibody (17A12) is available as the non-conjugated anti-PON1 antibody. Paroxon is an organophosphorus anticholinesterase compound, used topically in the treatment of glaucoma. It is produced in vivo in mammals by microsomal oxidation of the insecticide parathion. Parathion is inert until transformed to paroxon. Paroxonase or PON is an arylesterase that is capable of hydrolyzing paroxon to produce p-nitrophenol. PONs are nonspecific and their classification is based not only on substrate specificity but also on tissue distribution, inhibition properties and physicochemical characteristics such as electrophoretic mobility and molecular weight. In contrast to PON1, which is expressed mainly in the liver, PON2 is expressed in a variety of mouse tissues, including the pancreas. PON3 is a associated with the high density lipoprotein fraction of serum. The genes which encode PON1-3 are physically linked and map to human chromosome 7q21.3.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Id like to ask you about the blocking peptide for PON1 Antibody (17A12) is available because I tried to find it but I did not find it
Asked by: moamer
Thank you for your question. For this antibody no blocking peptide is available. Please contact our technical service to find out what other control options may be viable for your experiment.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06
I am using PON1 (17A12): sc-59646 antibody for immunoprecipitation, how much antibody should I use?
Asked by: TinTin
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here:
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