Anti-POLR2B Antibody (E-12) recommended for detection of POLR2B of mouse, rat, human and origin by WB, IP, IF and ELISA

Anti-POLR2B Antibody (E-12): sc-166803

Anti-POLR2B Antibody (E-12) is rated 5.0 out of 5 by 2.
  • y_2022, m_7, d_2, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.32
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_166803, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 116ms
  • REVIEWS, PRODUCT
loading
5
2
4
0
3
0
2
0
1
0

Datasheets

    • Anti-POLR2B Antibody (E-12) is a mouse monoclonal IgG2a κ POLR2B antibody, cited in 5 publications, provided at 200 µg/ml
    • raised against amino acids 184-384 mapping near the N-terminus of POLR2B of human origin
    • POLR2B Antibody (E-12) is recommended for detection of POLR2B of mouse, rat, human and origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
    • Anti-POLR2B Antibody (E-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-166803 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of POLR2B (E-12): sc-166803.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for POLR2B Antibody (E-12) for WB applications. These reagents are now offered in bundles with POLR2B Antibody (E-12) (see ordering information below).
loading

When should the TransCruz reagent sc-166803 X be used instead of the standard form sc-166803?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
  • y_2022, m_7, d_2, h_20CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.32
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_166803, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 122ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from great RPB2 antibody I tried this 1:5000 with Hela NE western, works great
Date published: 2016-11-30
Rated 5 out of 5 by from Satisfactory Western blot data of POLR2B Satisfactory Western blot data of POLR2B expression in HeLa nuclear extract. -SCBT QC
Date published: 2013-03-01
  • y_2022, m_7, d_2, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.32
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_166803, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 18ms
  • REVIEWS, PRODUCT