Pol III RPC39 Antibody (RPC39)recommended for detection of the RPC 39 subunit of RNA polymerase III of mouse, rat and human origin by WB, IP, IF and IHC(P)

Pol III RPC39 Antibody (RPC39): sc-21753

Pol III RPC39 Antibody (RPC39) is rated 5.0 out of 5 by 4.
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Datasheets

    • Pol III RPC39 Antibody (RPC39) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against recombinant human RPC39, subunit of RNA polymerase III
    • recommended for detection of the RPC 39 subunit of RNA polymerase III of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Can Pol III RPC39 (C39-1): sc-21753 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jerojero
Thank you for your question. Yes, Pol III RPC39 (C39-1): sc-21753 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Strong nuclear localization in IHC of formalin Strong nuclear localization in IHC of formalin-fixed human colon tissue. -SCBT QC
Date published: 2015-04-22
Rated 5 out of 5 by from Strong band in WB in 293T whole cell lysates Strong band in WB in 293T whole cell lysates. -SCBT QC
Date published: 2015-04-09
Rated 5 out of 5 by from Good specificity in WB in HeLa nuclear extract Good specificity in WB in HeLa nuclear extract. -SCBT QC
Date published: 2015-01-20
Rated 5 out of 5 by from Worked well in IHC in human nasopharyngeal Worked well in IHC in human nasopharyngeal carcinoma cell line. -SCBT Publication Review
Date published: 2013-09-07
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