Pol III RPC32 Antibody (C32-1)recommended for detection of RPC 32 subunit of RNA polymerase III of mouse, rat and human origin by WB, IP and IF

Pol III RPC32 Antibody (C32-1): sc-21754

Pol III RPC32 Antibody (C32-1) is rated 5.0 out of 5 by 4.
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Datasheets

    • Pol III RPC32 Antibody (C32-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against recombinant human RPC32 subunit of RNA polymerase III
    • Pol III RPC32 Antibody (C32-1) is recommended for detection of RPC 32 subunit of RNA polymerase III of mouse, rat and human origin by WB, IP and IF
    • Pol III RPC32 Antibody (C32-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Pol III RPC32 (C32-1): sc-21754.
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What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 2
Date published: 2017-02-08
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Rated 5 out of 5 by from Published for ChIP! Mouse monoclonal, Pol III RPC32 Antibody (C32-1) has been validated for ChIP in several publications, including a recent publication in Nature Communications (PMID: 26204128).
Date published: 2017-01-26
Rated 5 out of 5 by from Good antibody to use in ChIP in human HeLa Good antibody to use in ChIP in human HeLa cell line. -SCBT Publication Review
Date published: 2015-03-21
Rated 5 out of 5 by from Good specificity in WB in 293T whole cell lysates Good specificity in WB in 293T whole cell lysates. -SCBT QC
Date published: 2015-01-17
Rated 5 out of 5 by from Strong band in WB in HeLa whole cell lysates Strong band in WB in HeLa whole cell lysates. -SCBT QC
Date published: 2014-01-25
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