Pol II Antibody (mara-3)recommended for detection of Pol II of mouse, rat and human origin by WB and IP

Pol II Antibody (mara-3): sc-65884

Pol II Antibody (mara-3) is rated 3.0 out of 5 by 1.
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Datasheets

    • Pol II Antibody (mara-3) is a mouse monoclonal mouse IgM κ (kappa light chain) provided at 200 µg/ml
    • raised against the C-terminal domain of RNA Pol II of yeast origin
    • recommended for detection of Pol II of mouse, rat and human origin by WB and IP
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See Pol II (CTD4H8): sc-47701 for Pol II antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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I am using Pol II (mara-3): sc-65884 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Germaine
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 3 out of 5 by from Some background Some background, good bands observed by Western Blot using PC-12 and HeLa whole cell lysates. -SCBT QC
Date published: 2013-02-24
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