Pol II Antibody (F-12)recommended for detection of Pol II of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Pol II Antibody (F-12): sc-55492

Pol II Antibody (F-12) is rated 4.8 out of 5 by 8.
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Datasheets

    • Pol II Antibody (F-12) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-224 of Pol II of human origin
    • Pol II Antibody (F-12) is recommended for detection of Pol II of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Pol II Antibody (F-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Pol II (F-12): sc-55492.
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Is Pol II Antibody (F-12): sc-55492 available directly conjugated to HRP or fluorescent dyes?

Asked by: jenni
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at specialorders@scbt.com Alternatively, consider using Pol II (CTD4H8), which is available conjugated to agarose, HRP, FITC, PE, and more. (https://www.scbt.com/scbt/product/pol-ii-antibody-ctd4h8)
Answered by: Technical Support 4
Date published: 2017-01-09
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Rated 5 out of 5 by from Reliable tool for research! One of the main and most reliable antibodies for my research!
Date published: 2018-01-18
Rated 5 out of 5 by from Good for WB This antibody is good for Western blot with human cell nuclear extract.
Date published: 2017-12-30
Rated 4 out of 5 by from Okay antibody Didn't get clear bands like in the datasheet, rather got some smearing of signal.
Date published: 2017-08-22
Rated 5 out of 5 by from Superb !! Western blot and staining results are so much better than polyclonal alternatives
Date published: 2017-01-10
Rated 5 out of 5 by from Reliable Antibody for Many Applications This antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Very reliable.
Date published: 2016-12-22
Rated 5 out of 5 by from IF: good nuclear staining with HeLa cells IF: good nuclear staining with HeLa cells -SCBT QC
Date published: 2015-04-15
Rated 4 out of 5 by from Works well in WB with A Works well in WB with A-673 and NIH/3T3 nuclear extracts and AT-3 whole cell lysate -SCBT QC
Date published: 2015-03-10
Rated 5 out of 5 by from Great staining seen in IHC with paraffin Great staining seen in IHC with paraffin-embedded human rectal tissue -SCBT QC
Date published: 2014-11-13
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