Pol II Antibody (CTD4H8)recommended for detection of hypo- and hyper-phosphorylated Pol II of mouse, rat and human origin by WB, IP, IF and IHC(P)

Pol II Antibody (CTD4H8): sc-47701

Pol II Antibody (CTD4H8) is rated 4.4 out of 5 by 26.
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Datasheets

    • Pol II Antibody (CTD4H8) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-ser 5
    • Pol II Antibody (CTD4H8) is recommended for detection of hypo- and hyper-phosphorylated Pol II of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Pol II Antibody (CTD4H8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-47701 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Pol II (CTD4H8): sc-47701.
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With what kind of magnetic beads these Abs work good for ChIP? I tried with Dynabeads Protein G and I didn't get it

Asked by: Asya
Thank you for your question. We have not specifically tested this antibody using magnetic beads, and we do not sell those type of magnetic beads so we can not make a recommendation. This is a mouse monoclonal IgG1, so we would recommend asking the magnetic bead supplier for their recommendation. This specific monoclonal antibody has been validated for ChIP (please refer to the product citations for more details).
Answered by: Technical Support
Date published: 2019-01-26

According to the info sheet, this antibody was RNAPII Ser5p specific, have you check the specificity against Ser2p/Ser7p or non phopsphorylated RNAPII?

Asked by: Babybear
Thank you for your question. This antibody is recommended for detection of hypo- and hyper-phosphorylated Pol II of mouse, rat and human origin.
Answered by: Tech Service
Date published: 2019-01-22

Can I use this mouse monoclonal antibody for IHC or IF staining on mouse samples?

Asked by: Anna99
Yes, this Pol II Antibody (CTD4H8): sc-47701 is available conjugated to agarose (sc-47701 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-47701 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-47701 PE), fluorescein (sc-47701 FITC), Alexa Fluor® 488 (sc-47701 AF488) or Alexa Fluor® 647 (sc-47701 AF647), 200 µg/ml, for IF, IHC(P) and FCM.
Answered by: Tech Service 5
Date published: 2017-01-05
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Rated 5 out of 5 by from worked great at 1:1000 dilution the Western blot worked first time
Date published: 2019-11-01
Rated 4 out of 5 by from It works well for ChIP We tried this antibody for ChIP on both isolated primary mouse hepatocytes and mouse livers, and it worked well!
Date published: 2019-07-07
Rated 4 out of 5 by from Works for ChIP Thsi antibody Works well for Chip, for both cell culture and mouse livers.
Date published: 2019-05-07
Rated 5 out of 5 by from High Signal, no background pPolII antibody was tested on 20µg murine Chromatin. Antibody detected the correct band with no backgorund. We use the antibody 1:1000. No complains!
Date published: 2018-04-19
Rated 3 out of 5 by from Clean but low signal I got a trail size of this antibody and tested it on Murine erythroleukemia cells (MEL). I got clean sharp bands on Western blot, but the signal was very low. I didn't quantify the amount of protein, but the minimum lysate required to see a signal was roughly equivalent to that from 150,000 cells. IMO for Pol II that's a lot!
Date published: 2018-02-23
Rated 4 out of 5 by from Work for HEK cells CHIP! It worked for HEK cells CHIP experiment! It actually worked!
Date published: 2018-02-14
Rated 5 out of 5 by from good antibody This antibody works very well in ChIP-qPCR in MCF-7 and HeLa cells.
Date published: 2017-12-15
Rated 2 out of 5 by from Great for western blot This antibody works good for western blot when i used cell lysis from 293 cells.
Date published: 2017-12-06
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