Pol II Antibody (A-10)recommended for detection of Pol II of mouse, rat and human origin by WB, IP, IF and ELISA

Pol II Antibody (A-10): sc-17798

Pol II Antibody (A-10) is rated 4.7 out of 5 by 9.
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Datasheets

    • Pol II Antibody (A-10) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-224 of Pol II of human origin
    • Pol II Antibody (A-10) is recommended for detection of Pol II of mouse, rat and human origin by WB, IP, IF and ELISA
    • Pol II Antibody (A-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Pol II (A-10): sc-17798.
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I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Pol II Antibody (A-10): sc-17798?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 6
Date published: 2017-01-09
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Rated 3 out of 5 by from Works for WB This antibody works for Western blot at 1:200 dilution using human cell whole cell lysate. Not great.
Date published: 2018-02-21
Rated 5 out of 5 by from good antibody The antibody is well-quoted in a variety of applications with little background. In the observation process clearly, can be a good observation and guidance, and ultimately helped my test success
Date published: 2017-01-18
Rated 5 out of 5 by from Strong WB !! Very strong western blot data; also see CTD4H8 Clone
Date published: 2017-01-11
Rated 5 out of 5 by from Great antibody for Westerns This antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background.
Date published: 2016-12-30
Rated 4 out of 5 by from Used in WB Used in WB. Optimal results at a 1:300.
Date published: 2015-04-08
Rated 5 out of 5 by from Strong band in WB in A Strong band in WB in A-673, NIH/3T3 nuclear extracts and AT-3 whole cell lysates. -SCBT QC
Date published: 2015-03-30
Rated 5 out of 5 by from Good IP antibody in human LNCoP cells Good IP antibody in human LNCoP cells. -SCBT Publication Review
Date published: 2015-02-11
Rated 5 out of 5 by from Strong specificity in WB in human HepG2 cells Strong specificity in WB in human HepG2 cells. -SCBT Publication Review
Date published: 2015-01-05
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