Pol I/II/III RPB8 Antibody (B8-1)recommended for detection of RNA polymerase I/II/III subunit RPB8 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Pol I/II/III RPB8 Antibody (B8-1): sc-21752

Pol I/II/III RPB8 Antibody (B8-1) is rated 5.0 out of 5 by 2.
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Datasheets

    • Pol I/II/III RPB8 Antibody (B8-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against recombinant human RPB8, shared subunit of polymerase I, II and III
    • recommended for detection of RNA polymerase I/II/III subunit RPB8 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Can Pol I/II/III RPB8 (B8-1): sc-21752 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: AbPolly
Thank you for your question. Yes, Pol I/II/III RPB8 (B8-1): sc-21752 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Worked well in WB in Jurkat whole cell lysate Worked well in WB in Jurkat whole cell lysate. -SCBT QC
Date published: 2015-01-08
Rated 5 out of 5 by from Strong nuclear localization in IHC in formalin Strong nuclear localization in IHC in formalin-fixed human colon tissue. -SCBT QC
Date published: 2013-02-08
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