Pol I/II/III RPB6 Antibody (B6-1)recommended for detection of Pol I/II/III RPB6 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Pol I/II/III RPB6 Antibody (B6-1): sc-21751

Pol I/II/III RPB6 Antibody (B6-1) is rated 5.0 out of 5 by 3.
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Datasheets

    • Pol I/II/III RPB6 Antibody (B6-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against recombinant human RPB6, shared subunit of polymerase I, II and III
    • Pol I/II/III RPB6 Antibody (B6-1) is recommended for detection of Pol I/II/III RPB6 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Pol I/II/III RPB6 Antibody (B6-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Pol I/II/III RPB6 (B6-1): sc-21751.
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Can Pol I/II/III RPB6 (B6-1): sc-21751 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, Pol I/II/III RPB6 (B6-1): sc-21751 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Worked well in IF in human Hela cells Worked well in IF in human Hela cells. -SCBT Publication Review
Date published: 2015-04-05
Rated 5 out of 5 by from Strong nuclear localization in IHC in formalin Strong nuclear localization in IHC in formalin-fixed human breast carcinoma tissue. -SCBT QC
Date published: 2015-01-27
Rated 5 out of 5 by from Strong staining in IF in H1299 human lung Strong staining in IF in H1299 human lung carcinoma cells and MCF-7 human breast cancer cells. -SCBT Publication Review
Date published: 2013-03-21
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