Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Protein phosphatase methylesterase-1 (PME-1) catalyzes the demethylation and inactivation of protein phosphatase (PP2A), which is a multimeric phosphoserine/ threonine protein phosphatase associated with growth inhibition and cell cycle arrest. Carboxymethylation and demethylation is a covalent modifcation that regulates the catalytic activity of certain proteins in eukaryotes. Electrostatic interactions that occur at residues or metals in or near the active site can influence the specificity of carboxymethylation and demethylation. PME-1 can demethylate PP2A catalytic subunit in vitro and okadaic acid treatment is capable of inhibiting this reaction. PME-1 is conserved from yeast to human and contains a motif found in lipases having a catalytic triad-activated serine as their active site nucleophile.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
What is the recommended fixation method for immunofluorescence staining with PME-1 (F-6): sc-137162 monoclonal antibody?
Asked by: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here:
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