Anti-PLC gamma 1 Antibody (H-3) recommended for detection of PLC γ1 p145 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-PLC γ1 Antibody (H-3): sc-166938

Anti-PLC γ1 Antibody (H-3) is rated 4.3 out of 5 by 3.
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Datasheets

    • PLC γ1 Antibody (H-3) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 200 µg/ml
    • raised against amino acids 530-850 of PLC γ1 of rat origin
    • recommended for detection of PLC γ1 p145 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See PLC γ1 (E-12): sc-7290 for PLC γ1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PLC γ1 Antibody (H-3) for WB and IHC(P) applications. These reagents are now offered in bundles with PLC γ1 Antibody (H-3) (see ordering information below).
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Can PLC γ1 (H-3): sc-166938 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed
Thank you for your question. Yes, PLC γ1 (H-3): sc-166938 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Robust bands seen in WB using NIH/3T3 and Robust bands seen in WB using NIH/3T3 and KNRK whole cell lysates. -SCBT QC
Date published: 2015-03-06
Rated 4 out of 5 by from Publishable data in WB using mouse brain Publishable data in WB using mouse brain cell lines. -SCBT Publication Review
Date published: 2015-02-19
Rated 5 out of 5 by from Excellent immunofluorescence cytoplasmic Excellent immunofluorescence cytoplasmic staining in methanol-fixed NIH/3T3 cells. -SCBT QC
Date published: 2015-01-19
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