Anti-PLC γ1 Antibody (E-12) is a mouse monoclonal IgG3 κ PLC γ1 antibody, cited in 92 publications, provided at 200 µg/ml
specific for an epitope mapping between amino acids 1249-1262 near the C-terminus of PLC γ1 of bovine origin (differs from corresponding human sequence by a single amino acid)
recommended for detection of PLC γ1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine
available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; phycoerythrin, FITC, Alexa Fluor® 488 or Alexa Fluor® 647 for IF, IHC(P) and FCM
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PLC γ1 (E-12): sc-7290.
m-IgG3 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PLC γ1 Antibody (E-12) for WB and IHC(P) applications. These reagents are now offered in bundles with PLC γ1 Antibody (E-12) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
PLC γ1 Antibody (E-12) is a high quality monoclonal PLC gamma 1 antibody (also designated PLC gamma antibody, PLCG1 antibody, or phospholipase C gamma 1 antibody) suitable for the detection of the PLC gamma 1 protein of mouse, rat and human origin. PLC γ1 Antibody (E-12) is available as both the non-conjugated anti-PLC gamma 1 antibody form, as well as multiple conjugated forms of anti-PLC gamma 1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1, PLC δ2 and PLCε. PLC γ1 is widely distributed in bronchiolar epithelium, type I and II pneumocytes and fibroblasts of the interstitial tissue. Actin-regulatory protein Villin is tyrosine phosphorylated and associates with PLC γ1 in the brush border of intestinal epithelial cells. Villin regulates PLC γ1 activity by modifying its own ability to bind phosphatidylinositol 4,5-biphosphate. PLC γ1 binds Integrin α1/β1 and modulates Integrin α1/β-specific adhesion. PLC γ1 and Ca2+ play a direct role in VEGF-regulated endothelial growth, however this signaling pathway is not linked to FGF-mediated effects in primary endothelial cells. PLC γ1 is rapidly activated in response to growth factor stimulation and plays an important role in regulating cell proliferation and differentiation. It may also have a protective function during cellular response to oxidative stress.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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