Anti-PLC gamma 1 Antibody (E-12) recommended for detection of PLC γ1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-PLC γ1 Antibody (E-12): sc-7290

Anti-PLC γ1 Antibody (E-12) is rated 5.0 out of 5 by 15.
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Datasheets

    • Anti-PLC γ1 Antibody (E-12) is a mouse monoclonal IgG3 κ PLC γ1 antibody, cited in 92 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 1249-1262 near the C-terminus of PLC γ1 of bovine origin (differs from corresponding human sequence by a single amino acid)
    • recommended for detection of PLC γ1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; phycoerythrin, FITC, Alexa Fluor® 488 or Alexa Fluor® 647 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PLC γ1 (E-12): sc-7290.
    • m-IgG3 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PLC γ1 Antibody (E-12) for WB and IHC(P) applications. These reagents are now offered in bundles with PLC γ1 Antibody (E-12) (see ordering information below).
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What is the total volume provided of PLC γ1 (E-12): sc-7290 monoclonal antibody?

Asked by: jenni
Each vial contains 200 µg IgG3 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support 15
Date published: 2017-01-25
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Rated 5 out of 5 by from Works well for WB Used at 1:1000 in 3% BSA/TBST for WB and this antibody worked very well.
Date published: 2018-03-26
Rated 5 out of 5 by from It works well This product works well. The protein bands were observed clearly.
Date published: 2018-01-15
Rated 5 out of 5 by from high signal expression 1:800 dilution in WB, the specific results are no problem and the quality of good stability. To our satisfaction standard
Date published: 2017-10-26
Rated 5 out of 5 by from PLC γ1 is perfect in BMMC perfect band with low backgroud, and diluted as 1: 1000 for WB
Date published: 2017-06-30
Rated 5 out of 5 by from western blot I used this antibody in western blot and the band is very clear.
Date published: 2017-06-03
Rated 5 out of 5 by from An intense band of 155 kDa I used this antibody to produce a human breast cancer cell lysate; when diluted with 1:200, an intense band of 155 kDa was obtained.
Date published: 2017-05-17
Rated 5 out of 5 by from The one! Look no further for a well established monoclonal antibody specific for PLC gamma 1! The E-12 clone has been featured in over 50 publications for a variety of applications including WB, IF and IHC.
Date published: 2017-01-13
Rated 5 out of 5 by from Using this antibody makes me work well. I used it for western blotting. It worked very well, and I always got specific and clear band of 155kda.
Date published: 2016-12-21
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