Anti-PLC delta 4 Antibody (B-2) recommended for detection of PLC δ4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-PLC δ4 Antibody (B-2): sc-373875

Anti-PLC δ4 Antibody (B-2) is rated 3.7 out of 5 by 3.
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Datasheets

    • Anti-PLC δ4 Antibody (B-2) is a mouse monoclonal IgG1 κ PLC δ4 antibody, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 1-250 mapping at the N-terminus of PLC δ4 of human origin
    • Anti-PLC delta 4 Antibody (B-2) is recommended for detection of PLC δ4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-PLC delta 4 Antibody (B-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PLC δ4 (B-2): sc-373875.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PLC δ4 Antibody (B-2) for WB and IHC(P) applications. These reagents are now offered in bundles with PLC δ4 Antibody (B-2) (see ordering information below).
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I am using PLC δ4 (B-2): sc-373875 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenniferc
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Not working for my samples I used the polyclonal H-250 from Santa Cruz that was good to detect the correct MW ~ 88 kDa of this protein by WB, but it was discontinued. I tested this B2 side-by-side with the H-250 but the B2 is not working. I validate the H-250 in cells overexpressing and knockdown for PLCD4
Date published: 2019-05-01
Rated 5 out of 5 by from Exceptional immunoperoxidase cytoplasmic Exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human skeletal muscle tissue. -SCBT QC
Date published: 2015-02-04
Rated 5 out of 5 by from Signal via western blot analysis of PLC Signal via western blot analysis of PLC 4 expression in rat testis and rat skeletal muscle tissue extracts. -SCBT QC
Date published: 2015-01-27
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