Anti-PLC beta 4 Antibody (A-8) recommended for detection of PLC β4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-PLC β4 Antibody (A-8): sc-166131

Anti-PLC β4 Antibody (A-8) is rated 4.6 out of 5 by 7.
  • y_2021, m_11, d_28, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_en_US, sid_166131, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 117ms
  • REVIEWS, PRODUCT
loading
5
4
4
3
3
0
2
0
1
0

Datasheets

    • Anti-PLC β4 Antibody (A-8) is a mouse monoclonal IgG2a κ PLC β4 antibody, cited in 6 publications, provided at 200 µg/ml
    • raised against amino acids 876-1115 of PLC β4 of human origin
    • Anti-PLC beta 4 Antibody (A-8) is recommended for detection of PLC β4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-PLC beta 4 Antibody (A-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PLC β4 (A-8): sc-166131.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PLC β4 Antibody (A-8) for WB and IHC(P) applications. These reagents are now offered in bundles with PLC β4 Antibody (A-8) (see ordering information below).
loading

I am using PLC β4 (A-8): sc-166131 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenniferc
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2021, m_11, d_29, h_18CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_166131, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 117ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Excellent for IF! IF work very well (1:1000) on both embryonic and early postnatal mouse brain frozen section (PFA fixed overnight).
Date published: 2019-03-15
Rated 5 out of 5 by from very good! Sepecific bands were clearly shown with 1:500 dilution gentally shaking overnight.
Date published: 2017-05-03
Rated 5 out of 5 by from Beautiful staining! This is a great antibody for IHC and WB. Nice strong staining on paraffin sections and solid bands in WB.
Date published: 2017-01-17
Rated 4 out of 5 by from Robust bands seen in WB using mouse cerebellum Robust bands seen in WB using mouse cerebellum and rat cerebellum tissue extracts. -SCBT QC
Date published: 2015-03-29
Rated 4 out of 5 by from Strong band seen in WB using A549 whole cell Strong band seen in WB using A549 whole cell lysate. -SCBT QC
Date published: 2015-02-21
Rated 5 out of 5 by from Produced great immunoperoxidase cytoplasmic Produced great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human breast tissue. -SCBT QC
Date published: 2015-02-14
Rated 4 out of 5 by from Nice band seen in WB using ES Nice band seen in WB using ES-2 whole cell lysate. -SCBT QC
Date published: 2015-02-09
  • y_2021, m_11, d_28, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_en_US, sid_166131, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 31ms
  • REVIEWS, PRODUCT